Cd19 Mouse Monoclonal Antibody [Clone ID: MB19-1]

CAT#: AM08032PU-N

Cd19 mouse monoclonal antibody, clone MB19-1, Purified

Specifications

Product Data

• Clone Name: MB19-1
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry. (Ref.1-3)
  Immunoprecipitation. (Ref.1)
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgA
• Clonality: Monoclonal
• Immunogen: CD19+ mouse pre-B cell line, 300.19. (Ref.1)
• Specificity: This antibody is specific to CD19 B cell differentiation antigen (Mr. 95 kDa)
• Formulation: 100 mM Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD19 antigen
• Database Link:
  Entrez Gene 12478 Mouse
  UniProt ID P25918
• Background: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e., B220+/CD43+/HSA+) through fully differentiated B cell stages. (Ref.1-7) Terminally differentiated plasma cells do not express CD19.1 In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. (Ref.3,4) All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. (Ref.1,7) Recent studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. (Ref.7)
• Synonyms: Leu-12, B-cell marker