EIF4B Mouse Monoclonal Antibody [Clone ID: 1F5]

CAT#: AM06769PU-N

EIF4B mouse monoclonal antibody, clone 1F5, Purified

Product Images

Western blot analysis using EIF4B antibody against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)

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  Western blot analysis using EIF4B antibody against A549 (Lane 1), A431 (Lane 2), HepG2 (Lane 3), HEK293 (Lane 4), HeLa (Lane 5), Jurkat (Lane 6), K562 (Lane 7), NIH3T3 (Lane 8), and MCF-7 (Lane 9) cell lysate.

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  Red: Control Antigen (100 ng) Purple: Antigen (10 ng) Green: Antigen (50 ng) Blue: Antigen (100 ng)

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  Immunofluorescence analysis of NIH3T3 cells using EIF4B antibbody EIF4B (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of HeLa cells using EIF4B antibody (green) and Negative Control (red).

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Specifications

Product Data

• Clone Name: 1F5
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Bloting: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunocytochemistry: 1/200 - 1/1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human EIF4B expressed in E. Coli.
• Specificity: Recognizes EIF4B
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% protein stabilizer
  Preservative: 0.05% sodium azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 1975 Human
  UniProt ID P23588
• Background: The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA.It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F
• Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B