MCP1 (CCL2) Mouse Monoclonal Antibody [Clone ID: 2D8]

CAT#: AM06749PU-N

MCP1 (CCL2) mouse monoclonal antibody, clone 2D8, Purified

Product Images

Western blot analysis using CCL2 antibody against human CCL2 (AA: 1-99) recombinant protein (Expected MW is 36.5 kDa).

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  Western blot analysis using CCL2 antibody against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6)cell lysate.

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  Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CCL2 antibody with DAB staining.

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  Left: Immunofluorescence analysis of HepG2 cells. Right: Immunofluorescence analysis of HepG2 cells using CCL2 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of A549 cells using CCL2 antibody (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 2D8
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500-1/2000.
  Immunofluorescence: 1/200-1/1000.
  Flow Cytometry: 1/200-1/400.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CCL2 expressed in E.Coli.
• Specificity: Recognizes CCL2
• Formulation: PBS
  State: Purified
  State: Liquid purified antibody
  Stabilizer: 0.5% protein stabilizer
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 11 kDa
• Gene Name: C-C motif chemokine ligand 2
• Database Link:
  Entrez Gene 6347 Human
  UniProt ID P13500
• Background: This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
• Synonyms: C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11