Integrin beta 1 (ITGB1) Mouse Monoclonal Antibody [Clone ID: 3B6]

CAT#: AM06734PU-N

Integrin beta 1 (ITGB1) mouse monoclonal antibody, clone 3B6, Purified

Product Images

Western blot analysis using ITGB1 antibody against human ITGB1 (AA: 50-270) recombinant protein (Expected MW is 50.6 kDa).

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  Western blot analysis using ITGB1 antibody against Hela (1), HepG2 (2), A549 (3), Jurkat (4), L1210 (5) and Cos7 (6) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and cervical cancer tissues (right) using ITGB1 antibody with DAB staining.

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  Flow cytometric analysis of Hela cells using ITGB1 antibody (green) and negative control (red).

Specifications

Product Data

• Clone Name: 3B6
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human ITGB1 expressed in E. Coli.
• Specificity: Recognizes CD29 / Integrin beta-1
• Formulation: PBS
  State: Purified
  State: Liquid purified antibody
  Stabilizer: 0.5% protein stabilizer
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 88.4 kDa
• Gene Name: integrin subunit beta 1
• Database Link:
  Entrez Gene 3688 Human
  UniProt ID P05556
• Background: Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
• Synonyms: Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, ITGB1, FNRB, MDF2, MSK12