NEDD8 Mouse Monoclonal Antibody [Clone ID: 5B8]
| Product Data | |
| Clone Name | 5B8 |
|---|---|
| Application | ELISA, FC, IF, IHC, WB |
| Recommended Dilution | ELISA: 1/10000. Western Blot: 1/500-1/2000. Flow Cytometry: 1/200-1/400. Immunofluorescence: 1/200-1/1000. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Purified recombinant fragment of Human NEDD8 expressed in E. Coli. |
| Specificity | Recognizes NEDD8 |
| Buffer | PBS State: Purified State: Liquid purified antibody Stabilizer: 0.5% protein stabilizer Preservative: 0.05% Sodium Azide |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | 9 kDa |
| Gene Name | neural precursor cell expressed, developmentally down-regulated 8 |
| Database Link | |
| Background | Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. |
| Synonyms | APPBP1; FLJ43224; MGC104393; MGC125896; MGC125897; NAE1; Nedd-8; Neddylin |
| Reference Data | |
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