PPP2R4 (PTPA) Mouse Monoclonal Antibody [Clone ID: 4D9]

CAT#: AM06713PU-N

PPP2R4 (PTPA) mouse monoclonal antibody, clone 4D9, Purified

Product Images

Western blot analysis using PPP2R4 mAb against human PPP2R4 (AA: 1-154) recombinant protein. (Expected MW is 41 kDa)

• 

  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

• 

  Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP2R4 mouse mAb with DAB staining.

• 

  Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PPP2R4 mouse mAb with DAB staining.

• 

  Immunofluorescence analysis of Hela cells using PPP2R4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• 

  Flow cytometric analysis of MCF-7 cells using PPP2R4 mouse mAb (green) and negative control (red).

Show all 6 images

Specifications

Product Data

• Clone Name: 4D9
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human PPP2R4 expressed in E. Coli.
  
• Specificity: This antibody reacts to PPP2R4.
• Formulation: PBS
  State: Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.05% sodium azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 41 kDa
• Database Link:
  Entrez Gene 5524 Human
  UniProt ID Q15257
• Background: Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: PP2A subunit B' PR53 isoform, Phosphotyrosyl phosphatase activator