RPS6KA3 Mouse Monoclonal Antibody [Clone ID: 4E10]

CAT#: AM06696SU-N

RPS6KA3 mouse monoclonal antibody, clone 4E10, Ascites

Product Images

Western blot analysis using RSK2 mAb against human RSK2 (AA: 1-212) recombinant protein. (Expected MW is 49.7 kDa)

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  Western blot analysis using RSK2 mouse mAb against Hela (1), MCF-7 (2), and HepG2 (3) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of HepG2 cells using RSK2 mouse mAb (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 4E10
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human RSK2 expressed in E. Coli.
  
• Specificity: This antibody reacts to RSK2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 80 kDa
• Gene Name: ribosomal protein S6 kinase A3
• Database Link:
  Entrez Gene 6197 Human
  UniProt ID P51812
• Background: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).
• Synonyms: RSK-2, ISPK1, ISPK-1, MAPKAPK1, MAPKAPK-1b, S6K-alpha-3, p90RSK3, Ribosomal S6 kinase 2