c Kit (KIT) Mouse Monoclonal Antibody [Clone ID: 1C5]

CAT#: AM06670SU-N

c Kit (KIT) mouse monoclonal antibody, clone 1C5, Ascites

Product Images

Western blot analysis using KIT mAb against human KIT (AA: 805-976) recombinant protein. (Expected MW is 45 kDa)

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  Western blot analysis using KIT mouse mAb against Jurkat (1) and Hela (2) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded gastrointestinal stromal tumor using KIT mouse mAb with DAB staining.

Specifications

Product Data

• Clone Name: 1C5
• Applications: ELISA, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human KIT expressed in E. Coli.
  
• Specificity: This antibody reacts to KIT.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 145 kDa
• Gene Name: KIT proto-oncogene receptor tyrosine kinase
• Database Link:
  Entrez Gene 3815 Human
  UniProt ID P10721
• Background: The c-Kit proto-oncogene is a member of the receptor tyrosine kinase family and, more specifically, is closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene (v-Kit), encodes a transmembrane receptor. c-Kit regulates a variety of biological responses including chemotaxis, cell prolif- eration, apoptosis and adhesion. c-Kit is also identical with the product of the W locus in mice and, as such, is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and is encoded at the murine steel (SI) locus. Kit is the human homolog of the proto- oncogene c-Kit. Mutations in Kit are integral for tumor growth and progression in various cancers.
• Synonyms: SCFR, KIT