BLK Mouse Monoclonal Antibody [Clone ID: 1E6]

CAT#: AM06549SU-N

BLK mouse monoclonal antibody, clone 1E6, Ascites

Product Images

Western blot analysis using BLK antibody Cat.-No AM06549SU-N against HEK293 (1) and BLK (AA: 2-200)-hIgGFc transfected HEK293 (2) cell lysate.

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  Immunofluorescence analysis of Hela cells using BLK antibody Cat.-No AM06549SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled

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  Flow cytometric analysis of HL-60 cells using BLK antibody Cat.-No AM06549SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 1E6
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow Cytometry: 1/200 - 1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human BLK expressed in E. Coli.
• Specificity: Recognizes BLK
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 58 kDa
• Gene Name: BLK proto-oncogene, Src family tyrosine kinase
• Database Link:
  Entrez Gene 640 Human
  UniProt ID P51451
• Background: Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development . Activation of B cells by various ligands is accompanied by activation of Blk . It has been suggested that Blk is involved in the control of B cell differentiation and proliferation . Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis . Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response .
• Synonyms: B lymphocyte kinase, p55-BLK, p55BLK