TORC2 (CRTC2) Mouse Monoclonal Antibody [Clone ID: 5B10]

SKU AM06534SU-N

TORC2 (CRTC2) mouse monoclonal antibody, clone 5B10, Ascites

Product Data

• Clone Name: 5B10
• Application: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.; Western Blot: 1/500 - 1/2000.; Immunofluorescence: 1/200 - 1/1000.; Flow Cytometry: 1/200 - 1/400.; Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivity: Human, Monkey
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CRTC2 expressed in E. Coli.
• Specificity: Recognizes CRTC2 / TORC2
• Buffer: State: Ascites; State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. ; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Predicted Protein Size: 80 kDa
• Gene Name: CREB regulated transcription coactivator 2
• Database Link:
  Q53ET0
• Background: The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways . In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting. Tissue specificity: Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas .
• Synonyms: TORC-2

Reference Data