B7H3 (CD276) Mouse Monoclonal Antibody [Clone ID: 6A1]

CAT#: AM06515SU-N

B7H3 (CD276) mouse monoclonal antibody, clone 6A1, Ascites

Product Images

Western blot analysis using CD276 antibody Cat.-No AM06515SU-N against HEK293 (1) and CD276 (AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 antibody Cat.-No AM06515SU-N with DAB staining.

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  Flow Cytometric analysis of PC-3 cells using CD276 antibody Cat.-No AM06515SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 6A1
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CD276 expressed in E. Coli.
• Specificity: Recognizes CD276
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 57 kDa
• Gene Name: CD276 molecule
• Database Link:
  Entrez Gene 80381 Human
  UniProt ID Q5ZPR3
• Background: CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing.CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
• Synonyms: B7 homolog 3, 4Ig-B7-H3, PSEC0249