CD247 Mouse Monoclonal Antibody [Clone ID: 4B10]

CAT#: AM06414SU-N

CD247 mouse monoclonal antibody, clone 4B10, Ascites

Product Images

Western blot analysis using anti-CD247 antibody Cat.-No AM06414SU-N against CD247 (AA: 52-164)-hIgGFc transfected HEK293 cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-CD247 antibody Cat.-No AM06414SU-N

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  Immunofluorescence analysis of K562 cells using anti-CD247 antibody Cat.-No AM06414SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

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  Flow Cytometric analysis of Jurkat cells using anti-CD247 antibody Cat.-No AM06414SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 4B10
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human CD247 expressed in E. Coli.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 18 kDa
• Database Link:
  Entrez Gene 919 Human
  UniProt ID P20963
• Background: The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Synonyms: T3Z, TCRZ, CD3 zeta chain