PGA3 Mouse Monoclonal Antibody [Clone ID: 2C1]

CAT#: AM06373SU-N

PGA3 mouse monoclonal antibody, clone 2C1, Ascites

Product Images

Western blot analysis using PGA5 mouse mAb against HepG2 (Lane 1) and SMMC-7721 (Lane 2) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 mouse mAb with DAB staining.

Specifications

Product Data

• Clone Name: 2C1
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human PGA5 expressed in E. Coli.
• Specificity: This antibody recognizes Human PGA5. Other species not tested.
• Formulation: State: Ascites
  State: Ascitic fluid
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 42 kDa
• Database Link:
  Entrez Gene 643834 Human
  UniProt ID P0DJD8
• Background: PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor,pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates.PGA5 is inhibited by ovUS-1, a uterine serpin.
• Synonyms: Pepsin A, PGA, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I, Pepsinogen A, PGA3, PGA4, PGA5