Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: 8A5D12]

CAT#: AM06284SU-N

Cytokeratin 8 (KRT8) mouse monoclonal antibody, clone 8A5D12, Ascites

Product Images

Western blot analysis using Cytokeratin 8 antibody Cat.-No AM06284SU-N against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human Kidney tissues using Cytokeratin 8 antibody Cat.-No AM06284SU-N

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using Cytokeratin 8 antibody Cat.-No AM06284SU-N

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  Confocal Immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using Cytokeratin 8 antibody Cat.-No AM06284SU-N (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Product Data

• Clone Name: 8A5D12
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human Cytokeratin (aa391-483) expressed in E. Coli.
• Specificity: Recognizes Cytokeratin 8
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: keratin 8
• Database Link:
  Entrez Gene 3856 Human
  UniProt ID P05787
• Background: Cytokeratin 8, also known as CK8, KRT8, K8 is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
• Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A