JNK3 (MAPK10) Mouse Monoclonal Antibody [Clone ID: 10E4A4]

CAT#: AM06230SU-N

JNK3 (MAPK10) mouse monoclonal antibody, clone 10E4A4, Ascites

Product Images

Western blot analysis using MAPK10 antibody Cat.-No AM06230SU-N against NIH/3T3 (Lane 1) and SKN-SH (Lane 2) cell lysate.

• 

  Immunofluorescence staining of methanol-fixed A431 (left) and Hela (right) cells showing cytoplasmic and membrane localization.

Specifications

Product Data

• Clone Name: 10E4A4
• Applications: IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human MAPK10 (aa28-233) expressed in E. Coli.
• Specificity: Recognizes MAPK10
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase 10
• Database Link:
  Entrez Gene 5602 Human
  UniProt ID P53779
• Background: Mitogen-activated protein kinase 10, also known as JNK3, JNK3A, PRKM10, p54bSAPK. Entrez Protein NP_002744. It is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Synonyms: Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, JNK3A, PRKM10, MAP kinase p49 3F12