Apolipoprotein M (APOM) Mouse Monoclonal Antibody [Clone ID: 10C3G5]

CAT#: AM06158PU-N

Apolipoprotein M (APOM) mouse monoclonal antibody, clone 10C3G5, Purified

Product Images

Western blot analysis using ApoM antibody Cat.-No AM06158PU-N against GST-ApoM recombinant protein (1) and human serum (2).

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  Immunofluorescence analysis of methanol-fixed L-02 (left) and Cos7 (right) cells using ApoM antibody Cat.-No AM06158PU-N showing cytoplasmic and membrane localization.

Specifications

Product Data

• Clone Name: 10C3G5
• Applications: IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of ApoM expressed in E. Coli.
• Specificity: Recognizes ApoM (Apolipoprotein M).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 21 kDa
• Gene Name: apolipoprotein M
• Database Link:
  Entrez Gene 55937 Human
  UniProt ID O95445
• Background: ApoM (Apolipoprotein M, also designated G3a or NG20), with 188-amino acid protein(about 21kDa), is an apolipoprotein and member of the lipocalin protein family. The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro, Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment. Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs, and also found in triglyceride-rich lipoproteins and LDLs.
• Synonyms: Apo-M, ApoM, Protein G3A, NG20, HSPC336