RIT1 Mouse Monoclonal Antibody [Clone ID: 14G7]

CAT#: AM05290PU-N

RIT1 mouse monoclonal antibody, clone 14G7, Purified

Product Images

Figure 1. Western blot analysis using Rit antibody on control 293 cells (Lane 1), 293 cells expressing HA-tagged Rit protein (LAne 2) and 293 cells expressing HA-tagged Rin protein (Lane 3).

Specifications

Product Data

• Clone Name: 14G7
• Applications: WB
• Recommended Dilution: Western Blot (0.5-1 µg/ml).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human Rit protein and mouse myeloma cells.
• Specificity: This antibody detects recombinant Rit protein expressed in 293 cells.
  Does not cross-react with Rin protein.
• Formulation: 20 mM Sodium phosphate, 150 mM Sodium Chloride, 50% Glycerol
  at pH 7.5 and 3 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Ras like without CAAX 1
• Database Link:
  Entrez Gene 6016 Human
  UniProt ID Q92963
• Background: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64% amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100% conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.
• Synonyms: RIBB, RIT, ROC1