Calpain 1 (CAPN1) Mouse Monoclonal Antibody [Clone ID: 15C10]

CAT#: AM05266PU-N

Calpain 1 (CAPN1) mouse monoclonal antibody, clone 15C10, Purified

Specifications

Product Data

• Clone Name: 15C10
• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: ELISA: 0.5-1 µg/ml.
  Western blot: 0.5-1 µg/ml.
  Immunoprecipitation of both the native and denatured protein: 1-2 µg/ml.
  This Clone has been described to be suitable for Immunohistochemistry on Paraffin Sections.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Calpain purified from Human placenta.
• Specificity: This antibody (large subunit, p80) recognizes the 80 kD subunit of µ-Calpain as well as two smaller proteins that are presumed to be autolysis products.
• Formulation: 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50% Glycerol and 3 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction (> 95% pure).
• Concentration: lot specific
• Purification: Standard chromatographic techniques.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: calpain 1
• Database Link:
  Entrez Gene 823 Human
  UniProt ID P07384
• Background: The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar (mM) levels of calcium, µ-calpain is active at micromolar (µM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
• Synonyms: Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1
• Note: Predicted Molecular Weight: 80 kDa