AHNAK Mouse Monoclonal Antibody [Clone ID: EM-09]

SKU AM03134PU-N

AHNAK mouse monoclonal antibody, clone EM-09, Purified

Product Data

• Clone Name: EM-09
• Application: IF, IHC, IP, WB
• Recommended Dilution: Western Blot. ; Positive Control: HeLa Human cervix carcinoma cell line.; Immunocytochemistry (Permeabilization is required). ; Positive Control: HeLa Human cervix carcinoma cell line.; Immunoprecipitation. ; Immunohistochemistry on Frozen Sections.
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially expressed fragment of N-terminal domain of Human AHNAK1
• Specificity: The antibody reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
• Buffer: PBS, pH~7.4; State: Purified; State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE); Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.; DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: AHNAK nucleoprotein
• Database Link:
  Q09666
• Background: AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.
• Synonyms: Desmoyokin

Reference Data