CD69 Mouse Monoclonal Antibody [Clone ID: FN50]
CAT#: AM03132PU-N
CD69 mouse monoclonal antibody, clone FN50, Aff - Purified
Conjugation: APC FITC PE PerCP
Specifications
Product Data | |
Clone Name | FN50 |
Applications | FC, FN, IHC, WB |
Recommended Dilution | Flow Cytometry: 5 µg/ml. Western blot: 53 kDa, under reducing conditions it shows two bands of 29 and 32 kDa. Immunohistochemistry on Frozen Sections. Functional Assay: When stimulating peripheral blood T cells, FN50 antibody together with TPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheral blood B cells has similar, but weaker effect. |
Reactivities | Human |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Anti-µ-stimulated human B lymphocytes |
Specificity | The antibody recognizes CD69, an lymphocyte early activation marker detected as a 53 kDa band under nonreducing and as 29 + 32 kDa double band under reducing SDS-PAGE conditions. |
Formulation | PBS, pH~7.4 State: Aff - Purified State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Preservative: 15 mM Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Gene Name | CD69 molecule |
Database Link | |
Background | CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy. |
Synonyms | GP32/28, Leu-23, MLR-3, EA1, BL-AC/P26, AIM |
Reference Data |
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