Hsp60 (HSPD1) (1-573) Mouse Monoclonal Antibody [Clone ID: 2E4]

CAT#: AM03128PU-N

Hsp60 (HSPD1) (1-573) mouse monoclonal antibody, clone 2E4, Purified

Product Images

Western blot analysis : Cell lysates of Hela (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSP60 No. (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunohistochemistry : Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60No. (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Specifications

Product Data

• Clone Name: 2E4
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA.
  Western blot: 1/1,000-1/2,000.
  Immunohistochemistry on Paraffin Embedded Sections: 1/50-1/100 (following antigen retrieval and detection by DAB).
  Flow cytometry.
  Immunocytochemistry / Immunoflourescence.
  
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human HSP60 (1-573 aa) purified from E. coli
• Specificity: This antibody detects Human HSP60 at aa 1-573. Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: heat shock protein family D (Hsp60) member 1
• Database Link:
  Entrez Gene 3329 Human
  UniProt ID P10809
• Background: Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E.coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.
• Synonyms: HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog, CPN60