SOS1 Mouse Monoclonal Antibody [Clone ID: SOS-1]

CAT#: AM03036PU-N

SOS1 mouse monoclonal antibody, clone SOS-1, Aff - Purified

Product Images

Western Blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line;Lane 2: RAJI human Burkitt lymphoma cell line

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  Immunofluorescence staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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  Immunofluorescence staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

Specifications

Product Data

• Clone Name: SOS-1
• Applications: ELISA, IF, WB
• Recommended Dilution: Western Blotting under reducing conditions (1 µg/ml):
  Positive control: HeLa human cervix carcinoma cell line.
  Immunocytochemistry.
  ELISA.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.
• Specificity: The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Protein A affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid freeze/thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: SOS Ras/Rac guanine nucleotide exchange factor 1
• Database Link:
  Entrez Gene 20662 MouseEntrez Gene 6654 Human
  UniProt ID Q07889
• Background: The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.
• Synonyms: SOS-1, Son of sevenless homolog 1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway