HLAE (HLA-E) Mouse Monoclonal Antibody [Clone ID: MEM-E/07]
Product Data | |
Clone Name | MEM-E/07 |
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Application | FC, IHC, IP |
Recommended Dilution | Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 1-4 µg/ml |
Reactivity | Human |
Antibody Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide |
Specificity | The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly). |
Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Aff - Purified State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A chromatography (> 95% by SDS-PAGE) |
Conjugation | Unconjugated |
Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | major histocompatibility complex, class I, E |
Database Link | |
Background | HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. |
Synonyms | HLA-6.2, HLAE, MHC class I antigen E |
Reference Data |
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