MEK7 (MAP2K7) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10F7]

CAT#: AM00096PU-N

MEK7 (MAP2K7) (incl. pos. control) mouse monoclonal antibody, clone 10F7, Purified

Product Images

Detection of endogenous MKK7: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MKK7-10F7 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2

Specifications

Product Data

• Clone Name: 10F7
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 0.1 µg/ml.
  Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Positive Control: Cell lysate from Untreated SW480 cells.
  Immunocytochemistry.
  Immunohistochemistry on Frozen Sections.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
  Epitope: N-Terminus.
• Specificity: This antibody specifically recognizes the N-terminus of MKK7.
• Formulation: 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction from Serum-free Cell Culture Supernatant
• Reconstitution Method: Restore with 1 ml H₂O (15 min, RT).
• Purification: Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 45 kDa
• Gene Name: mitogen-activated protein kinase kinase 7
• Database Link:
  Entrez Gene 5609 Human
  UniProt ID O14733
• Background: The stress response of mammalian cells results in activation of kinases of the SAPK1 and SAPK2 families. The SAP kinases are specifically activated by dual phosphorylation of a conserved T-X-Y motif by MAP kinase kinases (MKK). The 3 isoforms of the SAPK1 family (SAPK1α/jnk2; SAPK1β/jnk3; SAPK1γ/jnk1) are synergistically activated by phosphorylation of the threonine residue by MKK7 and the tyrosine residue by MKK4.
• Synonyms: MAPK/ERK kinase 7, JNK-activating kinase 2, c-Jun N-terminal kinase kinase 2, MAPKK 7, NKK2, MEK7, MKK7, MAP kinase kinase 7
• Note: Protocol: Positive Control Cell lysate from untreated SW480 cells
  
  Format: Lyophilized cell lysate from serum starved SW480 cells.
  
  Reconstitution: Restore by addition of 200 μl H2O. After complete solubilization add 200 μl 2x SDSPAGE
  sample buffer, mix and incubate at 90°C for 5 min.
  
  Applications: The positive control cell lysate is recommended for immunoblot applications.
  20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 μl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
  
  Storage: In aliquots at -20°C. Avoid repeated freezing and thawing.