alpha 1 Catenin (CTNNA1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 15D9]

SKU AM00014PU-N

alpha 1 Catenin (CTNNA1) (incl. pos. control) mouse monoclonal antibody, clone 15D9, Purified

Product Data

• Clone Name: 15D9
• Application: ELISA, IF, IP, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.; Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; Included Positive Control: Cell lysate from untreated A431 cells.; ELISA: 0.05 µg/ml.; Immunoprecipitation: 1-10 µg per 10e6 vanadate-treated A431 cells.; Immunocytochemistry: 0.1-1 µg/ml. Mab Anti-Catenin Clone 15D9 may tolerate formaldehyd.; ; ; Positive Control Cell Lysate: A431 Untreated;
  • Format: Lyophilized cell lysate from serum starved A431 cells.
  • Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  • Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  • Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  • Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
  • Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.
• Reactivity: Canine, Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant α-Catenin
• Specificity: This antibody specifically recognizes α-Catenin in Western blot at 102 kDa.
• Buffer: 1 ml 2x PBS / 0.09% Sodium Azide / PEG and Sucrose; State: Purified; State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.; Avoid repeated freezing and thawing.; Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: catenin alpha 1
• Database Link:
  P35221
• Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The N-terminus of α-catenin directly interacts with exon 3 of β-catenin. The C-terminus of α-catenin interacts with the actin filament network.
• Synonyms: Alpha E-catenin, Alpha Catenin

Reference Data