beta Actin (ACTB) Goat Polyclonal Antibody

CAT#: AB0145-200

Goat Polyclonal Anti-beta-Actin Antibody

Product Images

Anti-beta-Actin Ab at 1/500 dilution; lysates at 100 ug per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

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  Immunofluorescence – anti-beta-Actin Ab at 1/100 dilution in NHI/3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin;

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:250-1:2,000; IF 1-50-1:250
• Reactivities: Canine, Human, Monkey, Mouse, Rat
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified recombinant peptide derived from within residues 100 aa to the N-terminus of human beta-Actin produced in E. coli.
• Specificity: Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC- 1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.
• Formulation: Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 3mg/ml
• Purification: epitope affinity purified
• Conjugation: Unconjugated
• Gene Name: actin, beta
• Database Link:
  NP_001092
  Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 574285 MonkeyEntrez Gene 60 Human
  UniProt ID P60709
• Synonyms: Beta-actin; PS1TP5BP1
• Note: Detects a band of approximately 42 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis