GAPDH Goat Polyclonal Antibody

CAT#: AB0049-500

Goat Polyclonal Anti-GAPDH Antibody

Product Images

Anti-GAPDH Ab at 1/2, 500 dilution; lysates at 50 ug per lane; Rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

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  Immunofluorescence – anti-GAPDH Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with methanol;

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  User supplied image. Magdalena D. for AB0049-200 on 01/20/2023: Used for Automatic Western Blot. 1:2000-1:30,000 with AntiGoat HRP 1:200. Band at 41kDa.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500-1:5,000; IF 1:50-1:250; IHC (paraffin) 1:200-1:1,000; IHC (frozen) 1:200-1:1,000
• Reactivities: Canine, Human, Monkey, Mouse, Rat, Zebrafish
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
• Specificity: Detects a band of 37 kDa by Western blot in the following Human (293A, HMEC-1, U- 118, HaCat), Rat (TR-iBRB), Mouse (AtT-20, Hepa), Canine (D17) and Monkey (COS-7) whole cell lysates.
• Formulation: Polyclonal antibody supplied as a 200 µl (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 2mg/ml
• Purification: epitope affinity purified
• Conjugation: Unconjugated
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 574353 MonkeyEntrez Gene 2597 Human
  UniProt ID P04406
• Background: Glyceraldehyde-3-phosphate dehydrogenase
• Synonyms: G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432
• Note: Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways