Specificity Validation Of OriGene Antibodies

Specificity is one of the most important attributes of antibodies. At OriGene, we have been working hard to validate specificities of our antibodies.  

Validation Methods

  • Predicted band detected in Western blot analysis
  • Independent antibody strategies
  • 10k protein chip
  • Western blot using Knockout cell lysates

Example Data

Specificity Validation for OriGene antibody Cat. No. UM500036
anti-her2 validation with over expression and endogenous lysates

Figure 1. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using human Her2 overexpression cell lysates and endogenous cell line lysates. UM500036 can recognize the specific Her2 protein at predicted size (~140KDa) in overexpression cell ysate (lane 1 ) and endogenous lysates prepared from Hela, A549 and MCF7 cell lines. The specificity is further proved by using an independent antibody (anti-DDK antibody in the middle) against the same recombinant overexpressed Her2 protein.

anti-her2 validation with 10k protein chip

Figure 2. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using 10k protein chip technology. UM500036 shows no cross-reactivity with other protein on the tested protein microarray containing >10,000 overexpression lysates for different human proteins.

anti-her2 validation with-ko cell ysate

Figure 3. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using Her2 knockout cell lysate. UM500036 recognizes the specific Her2 protein at correct size in wild type Hela cell lysate (WT lane), and shows cross-reactivity with any other protein in the double knockout cell lysates (KO lane).
WT: parental wild type Hela cell lysate
KO: Her2 double knockout Hela cell lysate

Antibody Resources