Specificity Validation of OriGene Antibodies

Specificity is one of the most important attributes of antibodies. As such, we have been working hard to validate specificities as well as sensitivities of our antibodies.

Validation Methods

Predicted band detected in Western blot analysis
Independent antibody strategies
10k protein chip
Western blot using Knockout cell lysates

Example Data

Specificity Validation for OriGene antibody Cat. No. UM500036

anti-her2 validation with over expression and endogenous lysates

Figure 1. Specificity validation of anti-HER2 antibody (Cat. no. UM500036) using human HER2 overexpression cell lysates and endogenous cell line lysates. UM500036 can recognize the specific HER2 protein at the predicted size (~140 kDa) in overexpression cell lysate (lane 1) and endogenous lysates prepared from HeLa, A549 and MCF7 cell lines. The specificity is further demonstrated by using an independent antibody (anti-DDK antibody in the middle) against the same recombinant overexpressed HER2 protein.

anti-her2 validation with 10k protein chip

Figure 2. Specificity validation of anti-HER2 antibody (Cat. no. UM500036) using 10K protein chip technology. UM500036 shows no cross-reactivity with other protein on the tested protein microarray containing >10,000 overexpression lysates for different human proteins.

anti-her2 validation with-ko cell ysate

Figure 3. Specificity validation of anti-HER2 antibody (Cat. no. UM500036) using HER2 knockout cell lysate. UM500036 recognizes the specific HER2 protein at the correct size in wild type HeLa cell lysate (WT lane), and does not show any cross-reactivity with other proteins in the -/- knockout cell lysates (KO lane). WT: parental wild type HeLa cell lysate. KO: HER2 -/- knockout HeLa cell lysate.