Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]
Applications | WB: 1: 1,000; ICC: 1:200; IP: 1:200 |
Reactivities | Mouse IgG |
Conjugation | HRP |
Immunogen | Mouse IgG, whole molecule |
Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]
Applications | WB: 1: 1,000; ICC: 1:200; IP: 1:200 |
Reactivities | Mouse IgG |
Conjugation | HRP |
Immunogen | Mouse IgG, whole molecule |
USD 865.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin
Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. General Recommended Dilutions: Immunocytochemistry. Immunohistochemistry (Paraffin). Histochemical Use: 1/10-1/40. ELISA: 1/100-1/2000. Western blot: 1/200-1/4000. |
Reactivities | Human |
Conjugation | Biotin |
IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified
Applications | ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16). |
Reactivities | Human |
Conjugation | AP |
USD 580.00
2 Weeks
Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites
Applications | To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
Reactivities | Human |
Conjugation | Unconjugated |
Mouse IgG (H+L chain) sheep polyclonal antibody, Aff - Purified
Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
Reactivities | Mouse |
Conjugation | Unconjugated |
Immunogen | Mouse IgG whole molecule. |
USD 865.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP
Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: ELISA: 1/20-1/500. Western Blot: 1/40-1/1000. Histochemical Use: 1/5-1/20. |
Reactivities | Human |
Conjugation | HRP |
Monkey IgG (Fc specific) goat polyclonal antibody, FITC
Applications | Can be used: • In direct staining of cytoplasmic IgG in fixed Monkey cells and tissue substrates. • To identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. • To identify a specific antigen or immune complex using a reference antibody of Monkey in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilution: 1/20-1/80. |
Reactivities | Monkey |
Conjugation | FITC |
Immunogen | Purified normal IgG isolated from pooled Rhesus Monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgG (H+L chain) goat polyclonal antibody, Aff - Purified
Applications | Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: IF Microscopy. ELISA: 1/10,000-1/60,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2500. Immunoprecipitation. |
Reactivities | Rabbit |
Conjugation | Unconjugated |
Immunogen | Rabbit IgG whole molecule |
Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified
Applications | Western blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections. Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland. |
Reactivities | Human |
Conjugation | Unconjugated |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
Reactivities | Mouse |
Conjugation | HRP |
Immunogen | Purified homogenous IgE isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG (Fc specific) goat polyclonal antibody, Aff - Purified
Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
Reactivities | Mouse |
Conjugation | Unconjugated |
Immunogen | Anti-Mouse IgG F(c) fragment was produced by repeated immunization with Mouse IgG F(c) fragment in goat. |
Monkey IgG (Fc specific) goat polyclonal antibody, HRP
Applications | Dot blot. Immunoblotting. ELISA. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. This antibody can be used: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histo- and Cytochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000. |
Reactivities | Monkey |
Conjugation | HRP |
Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
Reactivities | Monkey |
Conjugation | HRP |
Immunogen | Purified normal Fab of polyclonal Rhesus Monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin
Applications | Can be used in Immunocytochemical and Immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000 - 1/20000. |
Reactivities | Rabbit |
Conjugation | Biotin |
Immunogen | Purified normal IgG, IgA and IgM isolated from pooled rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA (Secretory component) goat polyclonal antibody, HRP
Applications | Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working dilutions: Histochemical and cytochemical 1/150 - 1/500. ELISA and comparable non-precipitating antibody-binding assays 1/200 -1/4000. |
Reactivities | Chimpanzee, Monkey |
Conjugation | HRP |
Immunogen | Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure. |