Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]
Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites
|Clone Name||NI 69 (A89-034) and NI 184 (A89-035)|
|Applications||ELISA, IF, IP, WB|
|Recommended Dilution||To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
|Immunogen||Highly purified monoclonal IgA isolated from pooled Human serum|
|Specificity||The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA.
It reacts with both subclasses of IgA as tested in Haemagglutination, Haemagglutination inhibition, indirect binding Enzyme Immunoassay, competitive Inhibition Enzyme Immunoassay, Immunoblotting, Immunoprecipitation, Latex Agglutination assay and Histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996). J. Immunol. Methods 193, 103-148).
Cross-Reactivity: The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
State: Lyophilized (Delipidated, heat inactivated) stable whole ascites without preservatives or foreign proteins.
|Reconstitution Method||Restore by adding 0.5 ml sterile distilled water.|
|Storage||Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
Avoid repeated freezing and thawing.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.