ORAI1 Human shRNA Plasmid Kit (Locus ID 84876)
ORAI1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector
|Product Name||ORAI1 Human shRNA Plasmid Kit (Locus ID 84876)|
|Synonyms||CRACM1; IMD9; ORAT1; TAM2; TMEM142A|
|Kit Components||ORAI1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 84876). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.|
|Summary||The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011].|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Evidence for STIM1- and Orai1-dependent store-operated calcium influx through ICRAC in vascular smooth muscle cells: role in proliferation and migration
,Marie Potier, José C. Gonzalez, Rajender K. Motiani, Iskandar F. Abdullaev, Jonathan M. Bisaillon, Harold A. Singer, and Mohamed Trebak,
FASEB J, Apr 2009; 10.1096/fj.09-131128.
Canonical Transient Receptor Potential 5 Channel in Conjunction with Orai1 and STIM1 Allows Sr2+ Entry, Optimal Influx of Ca2+, and Degranulation in a Rat Mast Cell Line
,Hong-Tao Ma, Ze Peng, Takaaki Hiragun, Shoko Iwaki, Alasdair M. Gilfillan, and Michael A. Beaven,
J. Immunol., Feb 2008; 180: 2233 - 2239.