IL2 (NM_000586) Human Recombinant Protein

CAT#: TP790117

Purified recombinant protein of Human interleukin 2 (IL2), Tag free, secretory expressed in CHO cells, 100ug

Product Images

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  Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.2~0.3 ng/mL. The specific activity of recombinant human IL-2 is >1.00 x 10 7 IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence from TrueORF clone, RC210013, encoding the region Ala21-Thr153 of IL2
• Tag: Tag Free
• Predicted MW: 15.4 kDa
• Concentration: >0.1 µg/µL as determined by microplate BCA method
• Purity: > 95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from a sterile solution containing PB, pH 7.4
• Endotoxin: < 1 EU per 1 µg of the protein by the LAL
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_000577
• Locus ID: 3558
• UniProt ID: P60568, Q0GK43
• Cytogenetics: 4q27
• Refseq Size: 822
• Refseq ORF: 459
• Synonyms: IL-2; lymphokine; TCGF
• Summary: This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Sep 2020]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus