CSK (NM_004383) Human Recombinant Protein

CAT#: TP310758

Recombinant protein of human c-src tyrosine kinase (CSK), transcript variant 1

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 Product Datasheet for 'TP310758'

USD 438

USD 730

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    • 20 ug

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Product Data
Description Recombinant protein of human c-src tyrosine kinase (CSK), transcript variant 1
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC210758. Click on the TrueORF clone link to view cDNA and protein sequences.
Predicted MW 50.5 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bioactivity CSK activity verified in a biochemical assay: CSK (c-src tyrosine kinase) (TP310758) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. CSK is a tyrosine kinase known to phosphorylate LCK, FYN and LYN. Varying concentrations of CSK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Reference Data
RefSeq NP_004374
Locus ID 1445
Refseq Size 2420
Cytogenetics 15q24.1
Refseq ORF 1350
Synonyms MGC117393
Protein Families
Protein Pathways
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