p53(TP53) (NM_000546) Human Mutant ORF Clone
TP53 mutant (R175H), Myc-DDK-tagged ORF clone of Homo sapiens tumor protein p53 (TP53), transcript variant 1 as transfection-ready DNA
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|Product Name||p53(TP53) (NM_000546) Human Mutant ORF Clone|
|Nucleotide Mutation||TP53 mutant (R175H), Myc-DDK-tagged ORF clone of Homo sapiens tumor protein p53 (TP53), transcript variant 1 as transfection-ready DNA|
|Synonyms||BCC7; LFS1; P53; TRP53|
|ORF Size||1182 bp|
>RC400040 representing NM_000546
Red=Cloning site Blue=ORF Green=Tags(s)
>RC400040 representing NM_000546
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Protein Families||Stem cell - Pluripotency, Transcription Factors, Druggable Genome|
|Protein Pathways||MAPK signaling pathway, Cell cycle, p53 signaling pathway, Apoptosis, Wnt signaling pathway, Neurotrophin signaling pathway, Amyotrophic lateral sclerosis (ALS), Huntington's disease, Pathways in cancer, Colorectal cancer, Pancreatic cancer, Endometrial cancer, Glioma, Prostate cancer, Thyroid cancer, Basal cell carcinoma, Melanoma, Bladder cancer, Chronic myeloid leukemia, Small cell lung cancer, Non-small cell lung cancer|
|Gene Summary||This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]|
|The use of this cDNA Clones has been cited in the following citations:|
Tumor Suppressor Protein p53 Negatively Regulates Human Pregnane X Receptor Activity
,Ayesha Elias, Jing Wu, and Taosheng Chen,
Mol. Pharmacol., Jun 2013; 83: 1229 - 1236.
,PubMed ID 23536728
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