p53 (TP53) (NM_000546) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC200003L4V

Lenti ORF particles, TP53 (mGFP-tagged) - Human tumor protein p53 (TP53), transcript variant 1, 200ul, >10^7 TU/mL

Product Images

RC200003L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC200003L4V particle to overexpress human TP53-mGFP fusion protein.

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  RC200003L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC200003L4V particle to overexpress human TP53-mGFP fusion protein.

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: TP53
• Synonyms: BCC7; BMFS5; LFS1; P53; TRP53
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_000546
• ORF Size: 1179 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC200003).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_000546.5
• RefSeq Size: 2591 bp
• RefSeq ORF: 1182 bp
• Locus ID: 7157
• UniProt ID: P04637
• Cytogenetics: 17p13.1
• Domains: P53
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway
• MW: 43.7 kDa
• Gene Summary: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]