DUS2L (DUS2) (NM_017803) Human Untagged Clone

CAT#: SC320857

DUS2L (untagged)-Human dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae) (DUS2L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUS2L
• Synonyms: DUS2L; SMM1; URLC8
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017803.3 TGGAGCACCGTGAGGAAGAAGCGAGGTTCTTTTTAAGAGTTCAGCTGCGAGATATCAAAC
  AAAGAATTACTCTGTACAAAGCCAGAACACATATATCAAAGTAATCCTGAAGTATCAGAA
  CAAAATAATAGGCTGTAACAGAGGAGGAAATGATTTTGAATAGCCTCTCTCTGTGTTACC
  ATAATAAGCTAATCCTGGCCCCAATGGTTCGGGTAGGGACTCTTCCAATGAGGCTGCTGG
  CCCTGGATTATGGAGCGGACATTGTTTACTGTGAGGAGCTGATCGACCTCAAGATGATTC
  AGTGCAAGAGAGTTGTTAATGAGGTGCTCAGCACAGTGGACTTTGTCGCCCCTGATGATC
  GAGTTGTCTTCCGCACCTGTGAAAGAGAGCAGAACAGGGTGGTCTTCCAGATGGGGACTT
  CAGACGCAGAGCGAGCCCTTGCTGTGGCCAGGCTTGTAGAAAATGATGTGGCTGGTATTG
  ATGTCAACATGGGCTGTCCAAAACAATATTCCACCAAGGGAGGAATGGGAGCTGCCCTGC
  TGTCAGACCCTGACAAGATTGAGAAGATCCTCAGCACTCTTGTTAAAGGGACACGCAGAC
  CTGTGACCTGCAAGATTCGCATCCTGCCATCGCTAGAAGATACCCTGAGCCTTGTGAAGC
  GGATAGAGAGGACTGGCATTGCTGCCATCGCAGTTCATGGGAGGAAGCGGGAGGAGCGAC
  CTCAGCATCCTGTCAGCTGTGAAGTCATCAAAGCCATTGCTGATACCCTCTCCATTCCTG
  TCATAGCCAACGGAGGATCTCATGACCACATCCAACAGTATTCGGACATAGAGGACTTTC
  GACAAGCCACGGCAGCCTCTTCCGTGATGGTGGCCCGAGCAGCCATGTGGAACCCATCTA
  TCTTCCTCAAGGAGGGTCTGCGGCCCCTGGAGGAGGTCATGCAGAAATACATCAGATACG
  CGGTGCAGTATGACAACCACTACACCAACACCAAGTACTGCTTGTGCCAGATGCTACGAG
  AACAGCTGGAGTCGCCCCAGGGAAGGTTGCTCCATGCTGCCCAGTCTTCCCGGGAAATTT
  GTGAGGCCTTTGGCCTTGGTGCCTTCTATGAGGAGACCACACAGGAGCTGGATGCCCAGC
  AGGCCAGGCTCTCAGCCAAGACTTCAGAGCAGACAGGGGAGCCAGCTGAAGATACCTCTG
  GTGTCATTAAGATGGCTGTCAAGTTTGACCGGAGAGCATACCCAGCCCAGATCACCCCTA
  AGATGTGCCTACTAGAGTGGTGCCGGAGGGAGAAGTTGGCACAGCCTGTGTATGAAACGG
  TTCAACGCCCTCTAGATCGCCTGTTCTCCTCTATTGTCACCGTTGCTGAACAAAAGTATC
  AGTCTACCTTGTGGGACAAGTCCAAGAAACTGGCGGAGCAGGCTGCAGCCATCGTCTGTC
  TGCGGAGCCAGGGCCTCCCTGAGGGTCGGCTGGGTGAGGAGAGCCCTTCCTTGCACAAGC
  GAAAGAGGGAGGCTCCTGACCAAGACCCTGGGGGCCCCAGAGCTCAGGAGCTAGCACAAC
  CTGGGGATCTGTGCAAGAAGCCCTTTGTGGCCTTGGGAAGTGGTGAAGAAAGCCCCCTGG
  AAGGCTGGTGACTACTCTTCCTGCCTTAGTCACCCCTCCATGGGCCTGGTGCTAAGGTGG
  CTGTGGATGCCACAGCATGAACCAGATGCCGTTGAACAGTTTGCTGGTCTTGCCTGGCAG
  AAGTTAGATGTCCTGGCAGGGGCCATCAGCCTAGAGCATGGACCAGGGGCCGCCCAGGGG
  TGGATCCTGGCCCCTTTGGTGGATCTGAGTGACAGGGTCAAGTTCTCTTTGAAAACAGGA
  GCTTTTCAGGTGGTAACTCCCCAACCTGACATTGGTACTGTGCAATAAAGACACCCCCTA
  CCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_017803
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017803.3, NP_060273.1
• RefSeq Size: 1999 bp
• RefSeq ORF: 1482 bp
• Locus ID: 54920
• UniProt ID: Q9NX74
• Cytogenetics: 16q22.1
• Domains: DSRM, Dus
• Gene Summary: This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).