DUS2L (DUS2) (NM_017803) Human Tagged ORF Clone

CAT#: RG200175

DUS2L (tGFP-tagged) - Human dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae) (DUS2L)

Product Images

Circular map for RG200175

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DUS2L
• Synonyms: DUS2L; SMM1; URLC8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200175 representing NM_017803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTTGAATAGCCTCTCTCTGTGTTACCATAATAAGCTAATCCTGGCCCCAATGGTTCGGGTAGGGA
  CTCTTCCAATGAGGCTGCTGGCCCTGGATTATGGAGCGGACATTGTTTACTGTGAGGAGCTGATCGACCT
  CAAGATGATTCAGTGCAAGAGAGTTGTTAATGAGGTGCTCAGCACAGTGGACTTTGTCGCCCCTGATGAT
  CGAGTTGTCTTCCGCACCTGTGAAAGAGAGCAGAACAGGGTGGTCTTCCAGATGGGGACTTCAGACGCAG
  AGCGAGCCCTTGCTGTGGCCAGGCTTGTAGAAAATGATGTGGCTGGTATTGATGTCAACATGGGCTGTCC
  AAAACAATATTCCACCAAGGGAGGAATGGGAGCTGCCCTGCTGTCAGACCCTGACAAGATTGAGAAGATC
  CTCAGCACTCTTGTTAAAGGGACACGCAGACCTGTGACCTGCAAGATTCGCATCCTGCCATCGCTAGAAG
  ATACCCTGAGCCTTGTGAAGCGGATAGAGAGGACTGGCATTGCTGCCATCGCAGTTCATGGGAGGAAGCG
  GGAGGAGCGACCTCAGCATCCTGTCAGCTGTGAAGTCATCAAAGCCATTGCTGATACCCTCTCCATTCCT
  GTCATAGCCAACGGAGGATCTCATGACCACATCCAACAGTATTCGGACATAGAGGACTTTCGACAAGCCA
  CGGCAGCCTCTTCCGTGATGGTGGCCCGAGCAGCCATGTGGAACCCATCTATCTTCCTCAAGGAGGGTCT
  GCGGCCCCTGGAGGAGGTCATGCAGAAATACATCAGATACGCGGTGCAGTATGACAACCACTACACCAAC
  ACCAAGTACTGCTTGTGCCAGATGCTACGAGAACAGCTGGAGTCGCCCCAGGGAAGGTTGCTCCATGCTG
  CCCAGTCTTCCCGGGAAATTTGTGAGGCCTTTGGCCTTGGTGCCTTCTATGAGGAGACCACACAGGAGCT
  GGATGCCCAGCAGGCCAGGCTCTCAGCCAAGACTTCAGAGCAGACAGGGGAGCCAGCTGAAGATACCTCT
  GGTGTCATTAAGATGGCTGTCAAGTTTGACCGGAGAGCATACCCAGCCCAGATCACCCCTAAGATGTGCC
  TACTAGAGTGGTGCCGGAGGGAGAAGTTGGCACAGCCTGTGTATGAAACGGTTCAACGCCCTCTAGATCG
  CCTGTTCTCCTCTATTGTCACCGTTGCTGAACAAAAGTATCAGTCTACCTTGTGGGACAAGTCCAAGAAA
  CTGGCGGAGCAGGCTGCAGCCATCGTCTGTCTGCGGAGCCAGGGCCTCCCTGAGGGTCGGCTGGGTGAGG
  AGAGCCCTTCCTTGCACAAGCGAAAGAGGGAGGCTCCTGACCAAGACCCTGGGGGCCCCAGAGCTCAGGA
  GCTAGCACAACCTGGGGATCTGTGCAAGAAGCCCTTTGTGGCCTTGGGAAGTGGTGAAGAAAGCCCCCTG
  GAAGGCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200175 representing NM_017803
  Red=Cloning site Green=Tags(s)
  
  MILNSLSLCYHNKLILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDD
  RVVFRTCEREQNRVVFQMGTSDAERALAVARLVENDVAGIDVNMGCPKQYSTKGGMGAALLSDPDKIEKI
  LSTLVKGTRRPVTCKIRILPSLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTLSIP
  VIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGLRPLEEVMQKYIRYAVQYDNHYTN
  TKYCLCQMLREQLESPQGRLLHAAQSSREICEAFGLGAFYEETTQELDAQQARLSAKTSEQTGEPAEDTS
  GVIKMAVKFDRRAYPAQITPKMCLLEWCRREKLAQPVYETVQRPLDRLFSSIVTVAEQKYQSTLWDKSKK
  LAEQAAAIVCLRSQGLPEGRLGEESPSLHKRKREAPDQDPGGPRAQELAQPGDLCKKPFVALGSGEESPL
  EGW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017803
• ORF Size: 1479 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017803.5
• RefSeq Size: 1999 bp
• RefSeq ORF: 1482 bp
• Locus ID: 54920
• UniProt ID: Q9NX74
• Cytogenetics: 16q22.1
• Domains: DSRM, Dus
• Gene Summary: This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]