FRA1 (FOSL1) (NM_005438) Human Untagged Clone

CAT#: SC125319

FOSL1 (untagged)-Human FOS-like antigen 1 (FOSL1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FRA1
• Synonyms: FRA; fra-1; FRA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125319 sequence for NM_005438 edited (data generated by NextGen Sequencing)
  ATGTTCCGAGACTTCGGGGAACCCGGCCCGAGCTCCGGGAACGGCGGCGGGTACGGCGGC
  CCCGCGCAGCCCCCGGCCGCAGCGCAGGCAGCCCAGCAGAAGTTCCACCTGGTGCCAAGC
  ATCAACACCATGAGTGGCAGTCAGGAGCTGCAGTGGATGGTACAGCCTCATTTCCTGGGG
  CCCAGCAGTTACCCCAGGCCTCTGACCTACCCTCAGTACAGCCCCCCACAACCCCGGCCA
  GGAGTCATCCGGGCCCTGGGGCCGCCTCCAGGGGTACGTCGAAGGCCTTGTGAACAGATC
  AGCCCGGAGGAAGAGGAGCGCCGCCGAGTAAGGCGCGAGCGGAACAAGCTGGCTGCGGCC
  AAGTGCAGGAACCGGAGGAAGGAACTGACCGACTTCCTGCAGGCGGAGACTGACAAACTG
  GAAGATGAGAAATCTGGGCTGCAGCGAGAGATTGAGGAGCTGCAGAAGCAGAAGGAGCGC
  CTAGAGCTGGTGCTGGAAGCCCACCGACCCATCTGCAAAATCCCGGAAGGAGCCAAGGAG
  GGGGACACAGGCAGTACCAGTGGCACCAGCAGCCCACCAGCCCCCTGCCGCCCTGTACCT
  TGTATCTCCCTTTCCCCAGGGCCTGTGCTTGAACCTGAGGCACTGCACACCCCCACACTC
  ATGACCACACCCTCCCTAACTCCTTTCACCCCCAGCCTGGTCTTCACCTACCCCAGCACT
  CCTGAGCCTTGTGCCTCAGCTCATCGCAAGAGTAGCAGCAGCAGCGGAGACCCATCCTCT
  GACCCCCTTGGCTCTCCAACCCTCCTCGCTTTGTGA
  
  Clone variation with respect to NM_005438.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005438 unedited
  NAAGTTAGGATTTGTATACGACTTCACTATAGGGCGGCCGCGAATCCCGGGNTATCGTCG
  ACCCACGCGTCCGCCCACGCGTCCGCAGAACCCAGCAGCCGTGTACCCCGCAGAGCCGCC
  AGCCCCGGGCATGTTCCGAGACTTCGGGGAACCCGGCCCGAGCTCCGGGAACGGCGGCGG
  GTACGGCGGCCCCGCGCAGCCCCCGGCCGCAGCGCAGGCAGCCCAGCAGAAGTTCCACCT
  GGTGCCAAGCATCAACACCATGAGTGGCAGTCAGGAGCTGCAGTGGATGGTACAGCCTCA
  TTTCCTGGGGCCCAGCAGTTACCCCAGGCCTCTGACCTACCCTCAGTACAGCCCCCCACA
  ACCCCGGCCAGGAGTCATCCGGGCCCTGGGGCCGCCTCCAGGGGTACGTCGAAGGCCTTG
  TGAACAGATCAGCCCGGAGGAAGAGGAGCGCCGCCGAGTAAGGCGCGAGCGGAACAAGCT
  GGCTGCGGCCAAGTGCAGGAACCGGAGGAAGGAACTGACCGACTTCCTGCAGGCGGAGAC
  TGACAAACTGGAAGATGAGAAATCTGGGCTGCAGCGAGAGATTGAGGAGCTGCAGAAGCA
  GAAGGAGCGCCTAGAGCTGGTGCTGGAAGCCCACCGACCCATCTGCAAAATCCCGGAAGG
  AGCCAAGGAGGGGGACACAGGCAGTACCAGTGGCACCAGCAGCCCACCAGCCCCCTGCCG
  CCCTGTACCTTGTATCTCCCTTTCCCCAGGGCCTGTGCTTGAACCTGAGGCACTGCACAC
  CCCCACACTCATGACCACACCCTCCCTAACTCCTTTCACCCCAGCCCTGGTCTCGACTAC
  CCCAGCACTCCTGAGCCTTGTGCCTCAGCTCAATCGCAGAGTAACAGCAGCAGCGGAGAC
  CATCCTCTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005438 unedited
  NGGACTTTTCAACCTTTTATTCATTTTGGTAGGTTTGTATAAAATACTCTCTTTAGGAAA
  ATAAATAGCAGCTGCCCTCGCTGTGGAGGCTTGGATTCCCTGGAGCTGAAGGCTTCTCAA
  AGCTGAGATCCGCAGATCAGCTCATCACAGAAGCCAAGGGCGCCTCCTGAGCTGTGCGGT
  TCAGGAGGGGGCGGCAGAATGGCCTGGTCCAATCACCTGCTGCTGCTGGCAGTGGGGCTG
  GTGAGTTAGTGTTCTAGGTGGGTAAAGTGGCACCTTCTGTCAGGAGATAGGGTTGGGTGG
  ATCACAGGAAGAGGGTGATGGAGAGTGTGGCAGTGATCTGGAAGGGGTTAGGGCTCCAGA
  GGACCTCTAAGGATCTACAAAGTCTCTGGGCTGCCAGGATTCCTCTGGCACAAATGGGAA
  ATATTTTGTCTCTGATTTAGTGCAAATTGTGCTAGAGAGGCCAGCTCAAGAGAAACAGTG
  GGCAGCTTTGGTGGCCCCAAGCTGGCTCTACTGTGAAGCACAATATGGTCAGTCTCATTA
  GAGGGATGGAGAAGAAGTTGCTGGAGTTGGATGTGGGATACTGTCCAGGCCAGCTGGACC
  GGTGGGGGAAGGGGAGGAGACATTGGCTAGGGTGGCATCTGCAGGGAGTAGGGCTCAGGC
  GCCTCACAAAGCGAGGAGGGTTGGAGAGCCAAGGGGGTCAGAGGATGGGTCTCCGCTGCT
  GCTGCTACTCTTGCGATGAGCTGAGGCACAAGGCTCAGGAGTGCTGGGGTAGGTGAAGAC
  CANGCTGGGGGTGAAAGGAGTTAGGGAGGGTGTGGTCATGANTGTGGNGGGTGTGCAGNG
  CCTCNGGTTCNGGCCCCGGCCCTTNGGTAAGGGTGATTCCCGGTTCATGGCTCGCNGGGT
  GCCTGGTGGGCTGCTGGTGCCCCTGGGACTGCACTGGTCCCCCAA
  
• Restriction Sites: Please inquire
• ACCN: NM_005438
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005438.2, NP_005429.1
• RefSeq Size: 1693 bp
• RefSeq ORF: 816 bp
• Locus ID: 8061
• UniProt ID: P15407
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Wnt signaling pathway
• Gene Summary: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).