NCF4 (NM_000631) Human Untagged Clone

CAT#: SC119769

NCF4 (untagged)-Human neutrophil cytosolic factor 4, 40kDa (NCF4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NCF4
• Synonyms: CGD3; NCF; P40PHOX; SH3PXD4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119769 sequence for NM_000631 edited (data generated by NextGen Sequencing)
  ATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGATGATGTT
  GCCATCTCGGCCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCACTTTGTT
  TTCGTCATCGAGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGCTACCGC
  CAGTTCCATGCTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAGAGCAGT
  GCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAACAGGAG
  ATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGCCTGCCG
  GTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTATGACTCA
  GAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAGAGCGTG
  TCCCCACAGGGCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTATTTGAC
  TTCACTGGAAACAGCAAACTGGAGCTGAATTTCAAAGCTGGAGATGTGATCTTCCTCCTC
  AGTCGGATCAACAAAGACTGGCTGGAGGGCACTGTCCGGGGAGCCACGGGCATCTTCCCT
  CTCTCCTTCGTGAAGATCCTCAAAGACTTCCCTGAGGAGGACGACCCCACCAACTGGCTG
  CGTTGCTACTACTACGAAGACACCATCAGCACCATCAAGGACATCGCGGTGGAGGAAGAT
  CTCAGCAGCACTCCCCTATTGAAAGACCTGCTGGAGCTCACAAGGCGGGAGTTCCAGAGA
  GAGGACATAGCTCTGAATTACCGGGACGCTGAGGGGGATCTGGTTCGGCTGCTGTCGGAT
  GAGGACGTAGCGCTCATGGTGCGGCAGGCTCGTGGCCTCCCCTCCCAGAAGCGCCTCTTC
  CCCTGGAAGCTGCACATCACGCAGAAGGACAACTACAGGGTCTACAACACGATGCCATGA
  
  Clone variation with respect to NM_000631.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000631 unedited
  AGGAATTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGCCCAAGGCAG
  CTCCTGGGGACTCCCAGACCACAGGCTGAGACGAGACGCAGGGTGGCTGGAGGAAGTGAG
  AGGTGAACTCAGCCTGGGACTGGCTGGGCGAGACTCTCCACCTGCTCCCTGGGACCATCG
  CCCACCATGGCTGTGGCCCAGCAGCTGCGGGCCGAGAGTGACTTTGAACAGCTTCCGGAT
  GATGTTGCCATCTCGGCCAACATTGCTGACATCGAGGAGAAGAGAGGCTTCACCAGCCAC
  TTTGTTTTCGTCATCGAGGTGAAGACAAAAGGAGGATCCAAGTACCTCATCTACCGCCGC
  TACCGCCAGTTCCATGCTTTGCAGAGCAAGCTGGAGGAGCGCTTCGGGCCAGACAGCAAG
  AGCAGTGCCCTGGCCTGTACCCTGCCCACACTCCCAGCCAAAGTCTACGTGGGTGTGAAA
  CAGGAGATCGCCGAGATGCGGATACCTGCCCTCAACGCCTACATGAAGAGCCTGCTCAGC
  CTGCCGGTCTGGGTGCTGATGGATGAGGACGTCCGGATCTTCTTTTACCAGTCGCCCTAT
  GACTCAGAGCAGGTGCCCCAGGCACTCCGCCGGCTCCGCCCGCGCACCCGGAAAGTCAAG
  AGCGTGTCCCCACAGGGCAACAGCGTTGACCGCATGGCAGCTCCGAGAGCAGAGGCTCTA
  TTTGACTTCACTGGAAACAGCAAACTGGAGCTGAATTTAAAGCTGGAGATGTGATCTTCC
  TNCTCAGTCGNATCAACNAGACTGGCTGGAGGGCACTGTCCGGNNGAGCCACGGCATCTT
  CCCTCTCTTCTTTCGTGAAGAACTCAAAGACTTCCCTGAGAGGACGACCCCNACACTGGC
  TGCGNTGCTACTACTACGAAGACACATCAGCACATCAGGACATCGCGGTGGAGGAAGACT
  CACAGACTNCCCTATGAANGACTGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000631 unedited
  TTTTTTTTTTTTTTTTTCCCCTTTCAAAGGAATGGTTTATTTAAATCAGATGTAAATAGT
  AGAAAGAGACAGATTACAAGCCCCACGGGTCCATTAGCCTCAAAAAAGGAAGTCTGCCCA
  TCCTCCCAGGTTTTCCTGGTCCCAATCTCCTCTGAGAGCTGAAGGTTTTTGCTGGTGTCC
  CCTCACTGCTCCAGGGACACCGTCAGCTCATGGCATCGTGTTGTAGACCCTGTAGTTGTC
  CTTCTGCGTGATGTGCAGCTTCCAGGGGAAGAGGCGCTTCTGGGAGGGGAGGCCACGAGC
  CTGCCGCACCATGAGCGCTACGTCCTCATCCGACAGCAGCCGAACCAGATCCCCCTCAGC
  GTCCCGGTAATTCAGAGCTATGTCCTCTCTCTGGAACTCCCGCCTTGTGAGCTCCAGCAG
  GTCTTTCAATAGGGGAGTGCTGCTGAGATCTTCCTCCACCGCGATGTCCTTGATGGTGCT
  GATGGTGTCTTCGTAGTAGTAGCAACGCAGCCAGTTGGTGGGGTCGTCCTCCTCAGGGAA
  GTCTTTGAGGATCTTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000631
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000631.3, NP_000622.2
• RefSeq Size: 1386 bp
• RefSeq ORF: 1020 bp
• Locus ID: 4689
• UniProt ID: Q15080
• Cytogenetics: 22q12.3
• Domains: PB1, SH3, PX
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2.