VAPB (NM_004738) Human Untagged Clone

CAT#: SC117187

VAPB (untagged)-Human VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAPB
• Synonyms: ALS8; VAMP-B; VAP-B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004738, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGCACGAGCTCAAATTCCGAGGTCCCTTCACCG
  ATGTTGTCACCACCAACCTAAAGCTTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTAC
  AGCACCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATCGATGCAGGGGCCTCAATTAATGTATCT
  GTGATGTTACAGCCTTTCGATTATGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGT
  TTGCTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTATGGATTC
  AAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAACCACATGATGTAGAAATAAATAAAATT
  ATATCCACAACTGCATCAAAGACAGAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTGGATGACA
  CCGAAGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGGTGAAGTTCAGAGGCTACGGGAGGAGAA
  CAAGCAGTTCAAGGAAGAAGATGGACTGCGGATGAGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCA
  TTAGCCCCAACTGGGAAGGAAGAAGGCCTTAGCACCCGGCTCTTGGCTCTGGTGGTTTTGTTCTTTATCG
  TTGGTGTAATTATTGGGAAGATTGCCTTGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004738 unedited
  GCTTTGCGGATTTGTAATCCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGC
  GCCCTCGCCCTCCGCCCCTGCGCCTGCACCGCGTAGACCGACCCCCCCCCCCATCGCGCC
  CACCCGGTAGAGGACCCCCGCCCGTGCCCCGACCGGTCCCCGCCTTTTTGTAAAACTTAA
  AGCGGGCGCAGCATTAACGCTTCCCGCCCCGGTGACCTCTCATGGGTCTCCCCGCCAAAG
  GTGCTCCGCCGCTAAGGAACATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGC
  ACGAGCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCACCAACCTAAAGCTTGGCA
  ACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTACAGCACCACGTAGGTACTGTG
  TGAGGCCCAACAGCGGAATCATCGATGCAGGGGCCTCAATTAATGTATCTGTGATGTTAC
  AGCCTTTCGATTATGATCCCAATGAGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGT
  TTGCTCCAACTGACACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACC
  TTATGGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAACCACATG
  ATGTAGAAATAAATAAAATTATATCCACAACTGCATCANAGACAGANACACCAATAGTGT
  CTAAGTCTCTGAGTTCTTCTTTGGATGACACCGAAAGTAAGAAAGTTATGGAAGAATGCT
  AGATGCTGNCAGGTGAGCTCANATGCTACGGGAGGAGAACAAGCAGTTCAAGGAAAGAGA
  TGGACTGCNGATGATGAAGACAGTGCAGAGCATCAGCCCCATTGCAGCATTAGCCCCCAC
  TGGGAAGGAAGAATGCCNNTANCACCCNGCTCTTGGCTCTGGTAGGGTTGGTCCTTATCG
  TAGGAGTATNTATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004738 unedited
  TTTATTATAATTTTTCTTTTCAGAATGCTACATAGTCAAACAGAACTGGGTTGGTCTTTT
  ATGGCATAAAATTAATTCACAGTCAAACTCTCAACTAGTACTAGAAGGATTCGCTGAAGA
  GTTTCTCTCTTCCCTTGCTCCCCACCCACAACTGCTGGTGCCCTCGCTTCTAACCCTCTG
  CAGCCCGATCCATTTGTCCTGACTCCAGGCTAGGTCCCCAAGGGGAGGTCAGGCTCAGAC
  TTGGACCTGGGCGCTGGAAGTGTGAGTAATGGTTGAGAGGTGGTATAAGAACAATCTAGA
  AGACTGACTACTCTTCATTAAAAAAGGTAACCTAGAGTTGACTGTCACTACTGAGAATTT
  CCCTCCCTATGACAGTTGTTATTTACTGACCCAGAAAAAACACTTCCTGGCAGCTCTTCT
  CCAGCATCCCAATAAACCCCACCTCCACATGACCAGACTCTCCAGAGACAGAGGAGCCCA
  CAGCCCTCCAGCAGCAATACCAGTTCACCCAACAGAGTTCAGCAGCACGCAGCATTAACT
  GTACTCTTGTGACAATAAGAAAACATGAGATGAAATACATTAAATAAGTTAAATATGCAT
  TAAACATCTCTGAATAACAGTTTGACTTCACTACATGAACCAATACAAATTTAGCTTGGT
  CCCTTTAAGTGAACAAAGCACTGCCATNTATTTCCAAAGCGCTGGGTCAATCAGTCAACA
  CACGCCTCTCACTTATGACGAGTACGTCTGATGCTGNCACAGTGCAATTCCTTTTGGCTT
  CTGACTCTGNTCATCAGTCCCAAGGAGCTGATGGCTTGGACAGAAACTGAGACAGGNAAA
  GCAGNCTGGCCCGGNAGTGGGCCACGTNNGGGACTACTGTGTGNAGCTGACCGNNACTCC
  CACATGCTGGGNCTCAGCTCCCCAGGGCCAGCACCACAGGCGAGGAGGGTTTCATTNTAG
  AGAGGTATCCAGCTCTGTACCAGGCCTACATGNTAAATGACNTACATTTGCATGNGGCAT
  ACTAAGGGTTCCCCTGCATATAAGACTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004738
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004738.3, NP_004729.1
• RefSeq Size: 3689 bp
• RefSeq ORF: 732 bp
• Locus ID: 9217
• UniProt ID: O95292
• Cytogenetics: 20q13.32
• Domains: MSP_domain
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.