TPX2 (NM_012112) Human Untagged Clone

CAT#: SC115515

TPX2 (untagged)-Human TPX2, microtubule-associated, homolog (Xenopus laevis) (TPX2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPX2
• Synonyms: C20orf1; C20orf2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; p100; REPP86
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012112 edited
  GAATTCGGCACGAGGGCGAGGCTAGGTGAGCCGTGGGAAGAAAAGAGGGAGCAGCTAGGG
  CGCGGGTCTCCCTCCTCCCGGAGTTTGGAACGGCTGAAGTTCACCTTCCAGCCCCTAGCG
  CCGTTCGCGCCGCTAGGCCTGGCTTCTGAGGCGGTTGCGGTGCTCGGTCGCCGCCTAGGC
  GGGGCAGGGTGCGAGCAGGGGCTTCGGGCCACGCTTCTCTTGGCGACAGGATTTTGCTGT
  GAAGTCCGTCCGGGAAACGGAGGAAAAAAAGAGTTGCGGGAGGCTGTCGGCTAATAACGG
  TTCTTGATACATATTTGCCAGACTTCAAGATTTCAGAAAAGGGGTGAAAGAGAAGATTGC
  AACTTTGAGTCAGACCTGTAGGCCTGATAGACTGATTAAACCACAGAAGGTGACCTGCTG
  AGAAAAGTGGTACAAATACTGGGAAAAACCTGCTCTTCTGCGTTAAGTGGGAGACAATGT
  CACAAGTTAAAAGCTCTTATTCCTATGATGCCCCCTCGGATTTCATCAATTTTTCATCCT
  TGGATGATGAAGGAGATACTCAAAACATAGATTCATGGTTTGAGGAGAAGGCCAATTTGG
  AGAATAAGTTACTGGGGAAGAATGGAACTGGAGGGCTTTTTCAGGGCAAAACTCCTTTGA
  GAAAGGCTAATCTTCAGCAAGCTATTGTCACACCTTTGAAACCAGTTGACAACACTTACT
  ACAAAGAGGCAGAAAAAGAAAATCTTGTGGAACAATCCATTCCGTCAAATGCTTGTTCTT
  CCCTGGAAGTTGAGGCAGCCATATCAAGAAAAACTCCAGCCCAGCCTCAGAGAAGATCTC
  TTAGGCTTTCTGCTCAGAAGGATTTGGAACAGAAAGAAAAGCATCATGTAAAAATGAAAG
  CCAAGAGATGTGCCACTCCTGTAATCATCGATGAAATTCTACCCTCTAAGAAAATGAAAG
  TTTCTAACAACAAAAAGAAGCCAGAGGAAGAAGGCAGTGCTCATCAAGATACTGCTGAAA
  AGAATGCATCTTCCCCAGAGAAAGCCAAGGGTAGACATACTGTGCCTTGTATGCCACCTG
  CAAAGCAGAAGTTTCTAAAAAGTACTGAGGAGCAAGAGCTGGAGAAGAGTATGAAAATGC
  AGCAAGAGGTGGTGGAGATGCGGAAAAAGAATGAAGAATTCAAGAAACTTGCTCTGGCTG
  GAATAGGGCAACCTGTGAAGAAATCAGTGAGCCAGGTCACCAAATCAGTTGACTTCCACT
  TCCGCACAGATGAGCGAATCAAACAACATCCTAAGAACCAGGAGGAATATAAGGAAGTGA
  ACTTTACATCTGAACTACGAAAGCATCCTTCATCTCCTGCCCGAGTGACTAAGGGATGTA
  CCATTGTTAAGCCTTTCAACCTGTCCCAAGGAAAGAAAAGAACATTTGATGAAACAGTTT
  CTACATATGTGCCCCTTGCACAGCAAGTTGAAGACTTCCATAAACGAACCCCTAACAGAT
  ATCATTTGAGGAGCAAGAAGGATGATATTAACCTGTTACCCTCCAAATCTTCTGTGACCA
  AGATTTGCAGAGACCCACAGACTCCTGTACTGCAAACCAAACACCGTGCACGGGCTGTGA
  CCTGCAAAAGTACAGCAGAGCTGGAGGCTGAGGAGCTCGAGAAATTGCAACAATACAAAT
  TCAAAGCACGTGAACTTGATCCCAGAATACTTGAAGGTGGGCCCATCTTGCCCAAGAAAC
  CACCTGTGAAACCACCCACCGAGCCTATTGGCTTTGATTTGGAAATTGAGAAAAGAATCC
  AGGAGCGAGAATCAAAGAAGAAAACAGAGGATGAACACTTTGAATTTCATTCCAGACCTT
  GCCCTACTAAGATTTTGGAAGATGTTGTGGGTGTTCCTGAAAAGAAGGTACTTCCAATCA
  CCGTCCCCAAGTCACCAGCCTTTGCATTGAAGAACAGAATTCGAATGCCCACCAAAGAAG
  ATGAGGAAGAGGACGAACCGGTAGTGATAAAAGCTCAACCTGTGCCACATTATGGGGTGC
  CTTTTAAGCCCCAAATCCCAGAGGCAAGAACTGTGGAAATATGCCCTTTCTCGTTTGATT
  CTCGAGACAAAGAACGTCAGTTACAGAAGGAGAAGAAAATAAAAGAACTGCAGAAAGGGG
  AGGTGCCCAAGTTCAAGGCACTTCCCTTGCCTCATTTTGACACCATTAACCTGCCAGAGA
  AGAAGGTAAAGAATGTGACCCAGATTGAACCTTTCTGCTTGGAGACTGACAGAAGAGGTG
  CTCTGAAGGCACAGACTTGGAAGCACCAGCTGGAAGAAGAACTGAGACAGCAGAAAGAAG
  CAGCTTGTTTCAAGGCTCGTCCAAACACCGTCATCTCTCAGGAGCCCTTTGTTCCCAAGA
  AAGAGAAGAAATCAGTTGCTGAGGGCCTTTCTGGTTCTCTAGTTCAGGAACCTTTTCAGC
  TGGCTACTGAGAAGAGAGCCAAAGAGCGGCAGGAGCTGGAGAAGAGAATGGCTGAGGTAG
  AAGCCCAGAAAGCCCAGCAGTTGGAGGAGGCCAGACTACAGGAGGAAGAGCAGAAAAAAG
  AGGAGCTGGCCAGGCTACGGAGAGAACTGGTGCATAAGGCAAATCCAATACGCAAGTACC
  AGGGTCTGGAGATAAAGTCAAGTGACCAGCCTCTGACTGTGCCTGTATCTCCCAAATTCT
  CCACTCGATTCCACTGCTAAACTCAGCTGTGAGCTGCGGATACCGCCCGGCAATGGGACC
  TGCTCTTAACCTCAAACCTAGGACCGTCTTGCTTTGTCATTGGGCATGGAGAGAACCCAT
  TTCTCCAGACTTTTACCTACCCGTGCCTGAGAAAGCATACTTGACAACTGTGGACTCCAG
  TTTTGTTGAGAATTGTTTTCTTACATTACTAAGGCTAATAATGAGATGTAACTCATGAAT
  GTCTCGATTAGACTCCATGTAGTTACTTCCTTTAAACCATCAGCCGGCCTTTTATATGGG
  TCTTCACTCTGACTAGAATTTAGTCTCTGTGTCAGCACAGTGTAATCTCTATTGCTATTG
  CCCCTTACGACTCTCACCCTCTCCCCACTTTTTTTAAAAATTTTAACCAGAAAATAAAGA
  TAGTTAAATCCTAAGATAGAGATTAAGTCATGGTTTAAATGAGGAACAATCAGTAAATCA
  GATTCTGTCCTCTTCTCTGCATACCGTGAATTTATAGTTAAGGATCCCTTTGCTGTGAGG
  GTAGAAAACCTCACCAACTGCACCAGTGAGGAAGAAGACTGCGTGGATTCATGGGGAGCC
  TCACAGCAGCCACGTAGCAGGCTCTGGGTGGGGCTGCCGTTAAGGCACGTTCTTTCCTTA
  CTGGTGCTGATAACAACAGGGAACCGTGCAGTGTGCATTTTAAGACCTGGCCTGGAATAA
  ATACGTTTTGTCTTTCCCTCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012112 unedited
  GAAGCACTTCGGATTTGTAAACCCGNATTCACTATNAGGGCGGACGCCAATTCGGCACGA
  GGGCGAGGCTAGGTGAGCCGTGGGAAGAAAAGAGGAGCAGCTAGGGCGCGGGTCTCCCTC
  CTCCCGGAGTTTGGAACGGCTGAAGTTCACCTTCCAGCCCCTAGCGCCGTTCGCGCCGCT
  AGGCCTGGCTTCTGAGGCGGTTGCGGTGCTCGGTCGCCTTTTATGCGGGGCAGGGTGCGA
  GCAGGGGCTTCGGGCCACGCTTCTCTTGGCGACAGGATTTTGCTGTGAAGTCCGTCCGGG
  AAACGGAGGAAAAAAAGAGTTGCGGGAGGCTGTCGGCTAATAACGGTTCTTGATACATAT
  TTGCCAGACTTCAAGATTTCAGAAAAGGGGTGAAAGAGAAGATTGCAACTTTGAGTCAGA
  CCTGTAGGCCTGATAGACTGATTAAACCACAGAAGGTGACCTGCTGAGAAAAGTGGTACA
  AATACTGGGAAAAACCTGCTCTTCTGCGTTAAGTGGGAGACAATGTCACAAGTTAAAAGC
  TCTTATTCCTATGATGCCCCCTCGGATTTCATCAATTTTTCATCCTTGGATGATGAAGGA
  GATACTCAAAACATAGATTCATGGTTTGAGGAGAAGGCCAATTTGGAGAATAAGTTACTG
  GGGAAGAATGGAACTGGAGGGCTTTTTCAGGGCAAAACTCCTTTGAGAAAGGCTAATCTT
  CAGCAAGCTATTGTCACACCTTTGAAACCAGTTGANCACACTTACTACAAAGAGGCAGAA
  AAAGAAAATCTTGTGGAACAATCCATTCCGTCAAATGCTTGTTCTTCCCTGGAAGTTTGA
  GCAGCCATATCAAGANAACTCCAGCCCAGCCTCAGAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012112 unedited
  CAGTGNAGATCGAGTTTTTTTTTTTTTTTTTTGAGGGAAAGACAAACGTATTTATTCCAG
  GCCAGGTCTTAAAATGCACACTGCACGGTTCCCTGTTGTTATCAGCACCAGTAAGGAAAG
  AACGTGCCTTAACGGCAGCCCCACCCAGAGCCTGCTACGTGGCTGCTGTGAGGCTCCCCA
  TGAATCCACGCAGTCTTCTTTCTCACTGGTGCAGTTGGTGAGGTTTTCTACCCTCACAGC
  AAAGGGATCCTTAACTATAAATTCACGGTATGCAGAGAAGAGGACAGAATCTGATTTACT
  GATTGTTCCTCATTTAAACCATGACTTAATCTCTATCTTAGGATTTAACTATCTTTATTT
  TCTGGTTAAAATTTTTAAAAAAAGTGGGGAGAGGGTGAGAGTCGTAAGGGGCAATAGCAA
  TAGAGATTACACTGTGCTGACACAGAGACTAAATTCTAGTCAGAGTGAAGACCCATATAA
  AAGGCCGGCTGATGGTTTAAAGGAAGTAACTACATGGAGTCTAATCGAGACATTCATGAG
  TTACATCTCATTATTAGCCTTAGTAATGTAAGAAAACAATTCTCAACAAAACTGGAGTCC
  ACAGTTGTCAAGTATGCTTTCTCAGGCACGGGTAGGTAAAAGTCTGGAGAAATGGGTTCT
  CTCCATGCCCAATGACAAAGCAAGACGGTCCTANGTTTGAGGTTAAGAGCANGTCCCATT
  GCCGGGCGGTATCCGCAGCTCACAGCTGAGTTACCAGTGAATCGAGTGGAGATTTGGGAG
  AACAGGACAGTCAGAGCTGTCACTTGACTTATCTCCAACCCTGTACTTGCCATTGGATTG
  CCTATGCACAGTTTTCTCGTAGCTGGCAGCTCTCTTTTCTGCCTCCTCCGGAGCGGCCTC
  CCACTGCTGGCTTTTGGCTTTACTAACAATTTTTTCAGTCTGCGGTTTGGTTTTTTAANA
  CCATGAAAGTCCGACCAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012112
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012112.4, NP_036244.2
• RefSeq Size: 3685 bp
• RefSeq ORF: 2244 bp
• Locus ID: 22974
• UniProt ID: Q9ULW0
• Cytogenetics: 20q11.21
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940).[UniProtKB/Swiss-Prot Function]