Butyrylcholinesterase (BCHE) (NM_000055) Human Untagged Clone

CAT#: SC115331

BCHE (untagged)-Human butyrylcholinesterase (BCHE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Butyrylcholinesterase
• Synonyms: BCHED; CHE1; CHE2; E1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115331 sequence for NM_000055 edited (data generated by NextGen Sequencing)
  ATGCATAGCAAAGTCACAATCATATGCATCAGATTTCTCTTTTGGTTTCTTTTGCTCTGC
  ATGCTTATTGGGAAGTCACATACTGAAGATGACATCATAATTGCAACAAAGAATGGAAAA
  GTCAGAGGGATGAACTTGACAGTTTTTGGTGGCACGGTAACAGCCTTTCTTGGAATTCCC
  TATGCACAGCCACCTCTTGGTAGACTTCGATTCAAAAAGCCACAGTCTCTGACCAAGTGG
  TCTGATATTTGGAATGCCACAAAATATGCAAATTCTTGCTGTCAGAACATAGATCAAAGT
  TTTCCAGGCTTCCATGGATCAGAGATGTGGAACCCAAACACTGACCTCAGTGAAGACTGT
  TTATATCTAAATGTATGGATTCCAGCACCTAAACCAAAAAATGCCACTGTATTGATATGG
  ATTTATGGTGGTGGTTTTCAAACTGGAACATCATCTTTACATGTTTATGATGGCAAGTTT
  CTGGCTCGGGTTGAAAGAGTTATTGTAGTGTCAATGAACTATAGGGTGGGTGCCCTAGGA
  TTCTTAGCTTTGCCAGGAAATCCTGAGGCTCCAGGGAACATGGGTTTATTTGATCAACAG
  TTGGCTCTTCAGTGGGTTCAAAAAAATATAGCAGCCTTTGGTGGAAATCCTAAAAGTGTA
  ACTCTCTTTGGAGAAAGTGCAGGAGCAGCTTCAGTTAGCCTGCATTTGCTTTCTCCTGGA
  AGCCATTCATTGTTCACCAGAGCCATTCTGCAAAGTGGATCCTTTAATGCTCCTTGGGCG
  GTAACATCTCTTTATGAAGCTAGGAACAGAACGTTGAACTTAGCTAAATTGACTGGTTGC
  TCTAGAGAGAATGAGACTGAAATAATCAAGTGTCTTAGAAATAAAGATCCCCAAGAAATT
  CTTCTGAATGAAGCATTTGTTGTCCCCTATGGGACTCCTTTGTCAGTAAACTTTGGTCCG
  ACCGTGGATGGTGATTTTCTCACTGACATGCCAGACATATTACTTGAACTTGGACAATTT
  AAAAAAACCCAGATTTTGGTGGGTGTTAATAAAGATGAAGGGACAGCTTTTTTAGTCTAT
  GGTGCTCCTGGCTTCAGCAAAGATAACAATAGTATCATAACTAGAAAAGAATTTCAGGAA
  GGTTTAAAAATATTTTTTCCAGGAGTGAGTGAGTTTGGAAAGGAATCCATCCTTTTTCAT
  TACACAGACTGGGTAGATGATCAGAGACCTGAAAACTACCGTGAGGCCTTGGGTGATGTT
  GTTGGGGATTATAATTTCATATGCCCTGCCTTGGAGTTCACCAAGAAGTTCTCAGAATGG
  GGAAATAATGCCTTTTTCTACTATTTTGAACACCGATCCTCCAAACTTCCGTGGCCAGAA
  TGGATGGGAGTGATGCATGGCTATGAAATTGAATTTGTCTTTGGTTTACCTCTGGAAAGA
  AGAGATAATTACACAAAAGCCGAGGAAATTTTGAGTAGATCCATAGTGAAACGGTGGGCA
  AATTTTGCAAAATATGGGAATCCAAATGAGACTCAGAACAATAGCACAAGCTGGCCTGTC
  TTCAAAAGCACTGAACAAAAATATCTAACCTTGAATACAGAGTCAACAAGAATAATGACG
  AAACTACGTGCTCAACAATGTCGATTCTGGACATCATTTTTTCCAAAAGTCTTGGAAATG
  ACAGGAAATATTGATGAAGCAGAATGGGAGTGGAAAGCAGGATTCCATCGCTGGAACAAT
  TACATGATGGACTGGAAAAATCAATTTAACGATTACACTAGCAAGAAAGAAAGTTGTGTG
  GGTCTCTAA
  
  Clone variation with respect to NM_000055.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000055 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATGATTTTCACTCCTTGCAAA
  CTTTGCCATCTTTGTTGCAGAGAATCGGAAATCAATATGCATAGCAAAGTCACAATCATA
  TGCATCAGATTTCTCTTTTGGTTTCTTTTGCTCTGCATGCTTATTGGGAAGTCACATACT
  GAAGATGACATCATAATTGCAACAAAGAATGGAAAAGTCAGAGGGATGAACTTGACAGTT
  TTTGGTGGCACGGTAACAGCCTTTCTTGGAATTCCCTATGCACAGCCACCTCTTGGTAGA
  CTTCGATTCAAAAAGCCACAGTCTCTGACCAAGTGGTCTGATATTTGGAATGCCACAAAA
  TATGCAAATTCTTGCTGTCAGAACATAGATCAAAGTTTTCCAGGCTTCCATGGATCAGAG
  ATGTGGAACCCAAACACTGACCTCAGTGAAGACTGTTTATATCTAAATGTATGGATTCCA
  GCACCTAAACCAAAAAATGCCACTGTATTGATATGGATTTATGGTGGTGGTTTTCAAACT
  GGAACATCATCTTTACATGTTTATGATGGCAAGTTTCTGGCTCGGGTTGAAAGAGTTATT
  GTAGTGTCAATGAACTATAGGGTGGGTGCCCTAGGATTCTTAGCTNTGCCAGGAAATCCT
  GAGGCTCCAGGGAACATGGGTTTATTTGATCAACAGTTGGCTCTTCAGTGGGTTCAAAAA
  AATATAGCAGCCTTTTGTGGAAATCCCTAAAGTGNTACTCTCTNTGGAGAAATGCANGAG
  CAGCTTCAGTTAGCCTGCATTTGCTTTCTCCTGNAAGCATTCATTGNTCACCAGANCCAT
  CTGCANAGTGGATCCTTTATGCTCCNTTGGCGGTACATCTCTTTATGAAGCTAGACAGAC
  GTTGAACTTACTAANTGACTGGTGCTCTAAAGATGAGCTGANTAATCAGGGCTAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000055 unedited
  TTTTAAAANNNNTTACTTGNACGCGGCCGCATTCTANGATCAGTTTTTTTTTTTTTTTTT
  TGATTTTCTGTGCTTATTTTAATATCTCATATTGACATTCAATTCTTATTTTAATTAGGA
  AACATAATATTGTAAACTATGGTGTACAGTGTTTCAATATTTAATTCATTTAAAAAAGAT
  ACCTTTTTACGAGTGGTAATGAAAATACACGTGACTAAAAGCAGAGCACTGATAATTTTG
  GGGGGAAAAAACTTAAATTTATTAAGGAAAGAAAGAAATTGAACCAGGCCCATTGTTTTA
  ATATGCACATAATTAACTGTAGAACTTTATATTGTGAAATTTAATTAAACATGTTCTAGC
  CATTTGGGTTTTTGAATGCTANGTAAAATACTAAGNTTAAAGATGTGAGGAATCAATATT
  ATCCTTCTGGCATTTTTGGTTCAGCTACATAATAACTTTTTTTAGTAGGTGTGTAAAAAA
  GCTCCTGATATTTTTGCCTTGATCTAAAGGAAAAATATGTCTATAAAAGGGTAANTCTAT
  TAATTANAGACCCACACCACTTTCTTTCTGGCTAGGGAAATCCGTAAATGATTTTTCCAG
  TCCATCATGTAATTGGTCCNAGGATTGAATCCTGCTTTTCACTCCCATTTGGTTTAATAA
  AATTTCCTGGCATTTCCAAAACTTTTGAAAAAATGAAGTCCAAAATCAAATGGTTAGCCC
  CGAATTTTCGCATTTTTCTTGGTGACCCTTGTATTAAGGGTAAAAATTTTTTGTAAAGGC
  TTTTAAAAACAGCCACTTGGGCCTTTGGTTTGAACCCATTTGGATTCCCATTTTTGCAAA
  TTTGCCCCCCTTTCCATTGAGATTACCCAAAATTCCCGCCTTTTGGGAAATAACCTTTTT
  TCCAGAGGAAACCAAAAAATTATTTTTTTAGCCTGCCTTTTCCCCCTCTTTTGGCCGAGA
  TTTGGGGGACGGGGTTAAAAATAAAAAAAGGTTTTTTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000055
• Insert Size: 2430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000055.1, NP_000046.1
• RefSeq Size: 2444 bp
• RefSeq ORF: 1809 bp
• Locus ID: 590
• UniProt ID: P06276
• Cytogenetics: 3q26.1
• Domains: COesterase
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (1) encodes the protein.