RAB18 (NM_021252) Human Untagged Clone

CAT#: SC112945

RAB18 (untagged)-Human RAB18, member RAS oncogene family (RAB18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAB18
• Synonyms: RAB18LI1; WARBM3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112945 sequence for NM_021252 edited (data generated by NextGen Sequencing)
  ATGGACGAGGACGTGCTAACCACCCTGAAGATCCTCATCATCGGCGAGAGTGGGGTGGGC
  AAGTCCAGCCTGCTCTTGAGGTTCACAGATGATACGTTTGATCCAGAACTTGCAGCAACA
  ATAGGTGTTGACTTTAAGGTGAAAACAATTTCAGTGGATGGAAATAAGGCTAAACTTGCA
  ATATGGGATACTGCTGGTCAAGAGAGGTTTAGAACATTAACTCCCAGCTATTATAGAGGT
  GCACAGGGTGTTATATTAGTTTATGATGTCACAAGAAGAGATACATTTGTTAAACTGGAT
  AATTGGTTAAATGAATTGGAAACATACTGTACAAGAAATGACATAGTAAACATGCTAGTT
  GGAAATAAAATCGATAAGGAAAATCGTGAAGTCGATAGAAATGAAGGCCTGAAATTTGCA
  CGAAAGCATTCCATGTTATTTATAGAGGCAAGTGCAAAAACCTGTGATGGTGTACAATGT
  GCCTTTGAAGAACTTGTTGAAAAGATCATTCAGACCCCTGGACTGTGGGAAAGTGAGAAC
  CAGAATAAAGGAGTCAAACTGTCACACAGGGAAGAAGGCCAAGGAGGAGGAGCCTGTGGT
  GGTTATTGCTCTGTGTTATAA
  
  Clone variation with respect to NM_021252.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021252 unedited
  GTCACGATATATGTATACGACTTACTATAGNNGGCGGCCGCGCAATTCGGCACGAGGCTC
  TGCTGAAGGCTGAGAGGCGCACCCGGGCGGCCAGCTGGGCTCGGAGCGGAACGGGGTCAG
  GATGGACGAGGACGTGCTAACCACCCTGAAGATCCTCATCATCGGCGAGAGTGGGGTGGG
  CAAGTCCAGCCTGCTCTTGAGGTTCACAGATGATACGTTTGATCCAGAACTTGCAGCAAC
  AATAGGTGTTGACTTTAAGGTGAAAACAATTTCAGTGGATGGAAATAAGGCTAAACTTGC
  AATATGGGATACTGCTGGTCAAGAGAGGTTTAGAACATTAACTCCCAGCTATTATAGAGG
  TGCACAGGGTGTTATATTAGTTTATGATGTCACAAGAAGAGATACATTTGTTAAACTGGA
  TAATTGGTTAAATGAATTGGAAACATACTGTACAAGAAATGACATAGTAAACATGCTAGT
  TGGAAATAAAATCGATAAGGAAAATCGTGAAGTCGATAGAAATGAAGGCCTGAAATTTGC
  ACGAAAGCATTCCATGTTATTTATAGAGGCAAGTGCAAAAACCTGTGATGGTGTACAATG
  TGCCTTTGAAGAACTTGTTGAAAAGATCATTCAGACCCCTGGACTGTGGGGAAGTGAGAA
  CCAGAATAAAGGAGTCAAACTGTCACACAGGNAAGAAGCCACGGACGAGGAGCCTGTGGT
  GGCTATTGCTCTGTGTTATAAACTCTGGGAAATTCCTCCTCTGCCATATTGATCAGAAGT
  GACCTCTTTCTGTATATAAACTCTTTACCGCTATTTTAGGAACCTTGCCAGTTGACCATT
  AATCGCTTCATATCACAACACGAAATATTTGCCAGAAATCCCACTCCTCGCCCCCGCGTT
  AAATGTCTATGTAAGCATGCCCACNTTGCAGTCTACAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021252 unedited
  TATGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTAGGGA
  AAGCAGGCTATATTTATTCAAGAACTAGAAAAGGTTACAATATAAATCTATCAAACAAAA
  TTCATTTTTGTTATATTCAAGTAACTCATATATATTCAAATTTAGACCAAATCAAGACAA
  CAATCCAATACACAGATTATTATATAGCTCAAGGAGCAAAAAACAACAGTCTTGTCAATA
  CAGACTCTACATAAATACACAAATCTCAAGTTTTAATATAACATGAACAAATCACAAGTA
  CATGTGTTAGTGTTCTTTCTCCAAATTTCTAGTAGTAAATCTTGTATATTTAGTAAAAGC
  ATATAGAATGTATAAAACCTTCAACCACATGTAATGTAGACTAGGAAGTGTAAATCAAGT
  AGAATGTGAGGTTTTTATATGTAAAGATGTACATTTCAGAAAGAACCCAACATGAAGTAA
  ATTAACAAACATATTGCAAAGGTGGTCACTCACAAAAATGCTTATCATTTGCAATTGTAT
  ACAGAGAACAAACTGCCCAGAAATTAATACTGGAATCTTACTGAGGGGTATTATAGTAAC
  CAACCTTAAAGGACAAATAACTTATTTGGGGGGTTAAAATAGCAGAGATCAGTGTAAATT
  AAATAACACATGAATGAAAAAAATCTACAATATACAATAGCAAGAGGTGAGAAAACTGAA
  TACTGTGAAAAAGATTTATTAGTCTGTACAAGTTGGGTTTGATGAACTACTGGTTACTTG
  TTAAGTAAATGGTCAAGAATTTCATTGTGACAGTATTTTCCCTCACACATCAGAATCTGG
  AAGCATATGCCTTTTTCAATTANACTTATGGTTTTTTTCCCACCCTCAAACACGAATAGG
  GTTTGATATCACTAGCCATTTGGAGCCGGAGAACCGAATTCTCACCTACCCAACTCAGTT
  AAGGCCTGGCACAACCTACAAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021252
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021252.3, NP_067075.1
• RefSeq Size: 2809 bp
• RefSeq ORF: 621 bp
• Locus ID: 22931
• UniProt ID: Q9NP72
• Cytogenetics: 10p12.1
• Domains: ras, RAN, RAS, RHO, RAB, ARF
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of a family of Ras-related small GTPases that regulate membrane trafficking in organelles and transport vesicles. Knockdown studies is zebrafish suggest that this protein may have a role in eye and brain development. Mutations in this gene are associated with Warburg micro syndrome type 3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.