HINT2 (NM_032593) Human Untagged Clone

CAT#: SC104681

HINT2 (untagged)-Human histidine triad nucleotide binding protein 2 (HINT2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HINT2
• Synonyms: HIT-17
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC104681 sequence for NM_032593 edited (data generated by NextGen Sequencing)
  ATGGCGGCAGCCGTGGTGCTGGCTGCTGGGTTGCGCGCGGCGCGCAGAGCCGTGGCGGCC
  ACGGGGGTGCGCGGGGGGCAGGTCCGAGGAGCTGCAGGTGTGACTGATGGGAATGAAGTG
  GCCAAGGCCCAGCAGGCAACTCCTGGGGGAGCAGCCCCAACCATCTTCTCCCGGATCCTG
  GACAAGAGCCTCCCAGCTGACATTCTCTATGAGGACCAGCAGTGTCTTGTGTTCCGTGAT
  GTGGCCCCTCAGGCTCCTGTGCACTTCCTGGTCATTCCTAAGAAGCCCATTCCTCGGATT
  AGCCAGGCTGAAGAAGAAGACCAGCAGCTTCTAGGACACCTACTCCTTGTGGCCAAGCAG
  ACAGCAAAGGCTGAGGGCCTGGGAGATGGATACCGACTTGTGATCAACGATGGGAAGCTG
  GGTGCACAATCTGTGTATCATCTGCACATTCATGTACTTGGGGGCCGGCAGCTCCAGTGG
  CCTCCAGGTTGA
  
  Clone variation with respect to NM_032593.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032593 unedited
  NNGGTCGACTTTTGTATACGACTCCTATAGGCGGCCGCGNATTCGGCACGAGGGCGCGCT
  CCATGGGGTAGCAGGGCTCGCCCGGCCGCCACTACCCCGCTTCCCCGCGCCCGGAGTCCC
  CACCCACGGCCGGCCGCGGAGCCGAGTGCTGACCCGGGTGAGAGGTTCCCGCGGCTCAGG
  GAAGATGGCGGCAGCCGTGGTGCTGGCTGCTGGGTTGCGCGCGGCGCGCAGAGCCGTGGC
  GGCCACGGGGGTGCGCGGGGGGCAGGTCCGAGGAGCTGCAGGTGTGACTGATGGGAATGA
  AGTGGCCAAGGCCCAGCAGGCAACTCCTGGGGGAGCAGCCCCAACCATCTTCTCCCGGAT
  CCTGGACAAGAGCCTCCCAGCTGACATTCTCTATGAGGACCAGCAGTGTCTTGTGTTCCG
  TGATGTGGCCCCTCAGGCTCCTGTGCACTTCCTGGTCATTCCTAAGAAGCCCATTCCTCG
  GATTAGCCAGGCTGAAGAAGAAGACCAGCAGCTTCTAGGACACCTACTCCTTGTGGCCAA
  GCAGACAGCAAAGGCTGAGGGCCTGGGAGATGGATACCGACTTGTGATCAACGATGGGAA
  GCTGGGTGCACAATCTGTGTATCATCTGCACANTTCATGTACTTGGGGGGCCGGCAGCTC
  CAGTGGCCTCCAGGTTGAACCTGCCAACTGATTAAAGGACACCAGACTCTGGATGCTTGG
  ATGGAAAGGGAAAAATGGACCCTGTGATGCTATNAAAACTGTTCTCCCTTANAAAAAAAA
  AAAAAAAAAAAAAAAACTCGACTCTANATTGCGGNCCGCGTCCATTAGCTGNTTCTGGAC
  AATCCCCCGGGGTGGCATCCCTGTGACCCCTNCCCATGCCTCTCTGGCCTTGGGGGAAGT
  TGCCACTCCAGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032593 unedited
  GAACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGG
  AGAACAGTTTTATTAGCATCACAGGGTCCATTTTTCCCTTTCCATCCAAGCATCCAGAGT
  CTGGTGTCCTTTAATCAGTTGGCAGGTTCAACCTGGAGGCCACTGGAGCTGCCGGCCCCC
  AAGTACATGAATGTGCAGATGATACACAGATTGTGCACCCAGCTTCCCATCGTTGATCAC
  AAGTCGGTATCCATCTCCCAGGCCCTCAGCCTTTGCTGTCTGCTTGGCCACAAGGAGTAG
  GTGTCCTAAAAGCTGCTGGTCTTCTTCTTCAGCCTGGCTAATCCGAGGAATGGGCTTCTT
  AGGAATGACCAGGAAGTGCACAGGAGCCTGAGGGGCCACATCACGGAACACAAGACACTG
  CTGGTCCTCATAGAGAATGTCAGCTGGGAGGCTCTTGTCCAGGATCCGGGAGAAGATGGT
  TGGGGCTGCTCCCCCAGGAGTTGCCTGCTGGGCCTTGGCCACTTCATTCCCATCAGTCAC
  ACCTGCAGCTCCTCGGACCTGCCCCCCGCGCACCCCCGTGGCCGCCACGGTTCTGCGCGC
  CGCGCGCAACCCAGCAGCCAGCACCACGGGCTGCCGCCATCTTCCCTGAGCCGCGGGAAC
  CTCTCACCCGGGTCAGCACTCGGCTCCGCGGCCCGCCGTGGGTGGGGACTCCGGGCGCGG
  GGAAANCGGGTANTGGCNGCCGGGCGAGCCCTGCTACCCCATTGGAGCGCGCCCTCGTGC
  CGAATTCGCGGNCGCCCTATAGTGAGTCGTATTACAAAATCTGACGGTCACTAACGAGCT
  CTGCTATATAGACTNNCACGTACACGCTACCGCCATTGCGTAACGGGGCGGGTATTACGA
  ATTTGNAAAGNCCGTGATTTTGTGCAAACAACTCCATGACTNATGGGGTGAACTGAAATC
  CNGGAGCAACGCTTCCAGCCATGGTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032593
• Insert Size: 820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032593.2, NP_115982.1
• RefSeq Size: 652 bp
• RefSeq ORF: 492 bp
• Locus ID: 84681
• UniProt ID: Q9BX68
• Cytogenetics: 9p13.3
• Domains: HIT
• Gene Summary: Histidine triad proteins, such as HINT2, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM, Mar 2008]