ZNF182 (NM_001178099) Human Tagged ORF Clone

CAT#: RG230383

ZNF182 (tGFP-tagged) - Human zinc finger protein 182 (ZNF182), transcript variant 3. Note: ORF is codon optimized

Product Images

Circular map for RG230383

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF182
• Synonyms: HHZ150; KOX14; Zfp182; ZNF21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230383 ORF sequence, codon optimized.
  Due to the complexity of NM_001178099, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCCCGCCTCTGCCTCCGGCGAGGATTCTGGCTCCTTTTATAGCTGGCAAAAGGCAAAGCGGGAGC
  AGGGACTTGTTACTTTCGAGGATGTTGCGGTCGATTTCACTCAGGAGGAGTGGCAGTACCTGAACCCCCC
  CCAGAGAACTCTTTATAGGGATGTGATGCTTGAGACATACTCCAATCTGGTCTTTGTAGGACAGCAGGTC
  ACCAAGCCGAACCTGATCCTTAAATTGGAAGTGGAGGAGTGCCCCGCCGAGGGTAAAATTCCCTTTTGGA
  ACTTTCCAGAAGTTTGCCAAGTGGACGAGCAGATTGAGAGACAGCACCAGGACGATCAGGACAAGTGTCT
  CCTGATGCAGGTCGGATTCTCAGACAAAAAAACAATCATCACGAAGAGCGCACGCGACTGTCACGAATTT
  GGAAACATTCTGCACCTGAGTACCAACCTGGTCGCATCCATCCAGCGGCCCGACAAGCATGAATCCTTTG
  GGAATAATATGGTTGACAATTTGGACCTCTTTTCCAGGTCAAGTGCAGAAAACAAATATGACAACGGGTG
  CGCCAAGCTGTTCTTCCATACGGAGTATGAGAAGACCAACCCGGGGATGAAGCCTTATGGTTACAAGGAG
  TGTGGAAAGGGATTGAGGCGGAAGAAGGGGCTCTCACTGCACCAGAGAATTAAGAACGGCGAAAAACCAT
  TTGAGTGTACCGCTTGCCGAAAAACTTTCTCTAAGAAAAGTCATCTGATAGTGCACTGGAGAACTCACAC
  CGGAGAAAAACCGTTCGGCTGCACCGAGTGTGGCAAGGCGTTCTCTCAGAAATCCCAGTTGATCATACAT
  CTCCGCACCCATACCGGCGAACGCCCTTTCGAATGTCCCGAGTGCGGTAAGGCTTTCCGCGAGAAATCCA
  CTGTCATCATTCATTATCGGACACATACCGGCGAGAAACCATATGAATGTAACGAATGTGGAAAGGCCTT
  TACACAGAAAAGTAATCTCATCGTGCACCAGAAGACCCACACTGGGGAAAAAACTTACGAGTGTACGAAA
  TGTGGGGAGTCATTTATTCAGAAGCTCGACTTGATTATACATCATAGTACTCACACTGGGAAAAAGCCGC
  ACGAGTGTAATGAATGTAAGAAAACCTTCAGCGATAAATCAACTCTGATCATTCATCAGCGGACACACAC
  CGGCGAGAAGCCACACAAGTGCACAGAATGCGGCAAGAGCTTTAACGAAAAAAGTACACTGATTGTTCAT
  CAAAGAACACACACCGGAGAAAAACCCTATGAGTGTGACGTGTGCGGAAAAACATTCACACAGAAGTCCA
  ATCTTGGCGTGCATCAGAGGACACATAGCGGCGAGAAACCTTTCGAATGTAATGAGTGTGAAAAGGCCTT
  TAGCCAGAAGAGCTATCTTATGCTCCATCAGCGCGGCCACACGGGTGAGAAACCATACGAGTGCAATGAA
  TGTGAGAAAGCTTTTAGTCAGAAGAGCTACTTGATCATTCATCAGAGAACGCATACAGAAGAGAAGCCAT
  ACAAGTGCAACGAGTGTGGTAAGGCTTTCAGAGAGAAGAGCAAACTTATTATCCATCAGCGAATTCACAC
  AGGGGAGAAACCCTATGAATGCCCAGTCTGTTGGAAAGCATTTTCACAGAAATCTCAGCTTATTATTCAC
  CAGAGAACCCATACAGGCGAGAAACCATATGCCTGTACAGAATGCGGCAAGGCCTTCCGCGAGAAGTCCA
  CGTTCACCGTACACCAGAGAACTCATACAGGCGAAAAACCCTATAAATGCACCGAATGCGGGAAGGCTTT
  TACACAGAAGTCAAACCTCATAGTGCACCAGAGGACGCATGCCGGCAAGAAGGCGCACGGGCGGGGACAT
  ACCCGAAAGAGTAAGTTCATGGCTCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230383 representing NM_001178099
  Red=Cloning site Green=Tags(s)
  
  MTPASASGEDSGSFYSWQKAKREQGLVTFEDVAVDFTQEEWQYLNPPQRTLYRDVMLETYSNLVFVGQQV
  TKPNLILKLEVEECPAEGKIPFWNFPEVCQVDEQIERQHQDDQDKCLLMQVGFSDKKTIITKSARDCHEF
  GNILHLSTNLVASIQRPDKHESFGNNMVDNLDLFSRSSAENKYDNGCAKLFFHTEYEKTNPGMKPYGYKE
  CGKGLRRKKGLSLHQRIKNGEKPFECTACRKTFSKKSHLIVHWRTHTGEKPFGCTECGKAFSQKSQLIIH
  LRTHTGERPFECPECGKAFREKSTVIIHYRTHTGEKPYECNECGKAFTQKSNLIVHQKTHTGEKTYECTK
  CGESFIQKLDLIIHHSTHTGKKPHECNECKKTFSDKSTLIIHQRTHTGEKPHKCTECGKSFNEKSTLIVH
  QRTHTGEKPYECDVCGKTFTQKSNLGVHQRTHSGEKPFECNECEKAFSQKSYLMLHQRGHTGEKPYECNE
  CEKAFSQKSYLIIHQRTHTEEKPYKCNECGKAFREKSKLIIHQRIHTGEKPYECPVCWKAFSQKSQLIIH
  QRTHTGEKPYACTECGKAFREKSTFTVHQRTHTGEKPYKCTECGKAFTQKSNLIVHQRTHAGKKAHGRGH
  TRKSKFMAH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178099
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178099.1, NP_001171570.1
• RefSeq Size: 3669 bp
• RefSeq ORF: 1920 bp
• Locus ID: 7569
• UniProt ID: P17025
• Cytogenetics: Xp11.23
• Gene Summary: Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This gene encodes a zinc finger protein, and belongs to the krueppel C2H2-type zinc-finger protein family. It may be involved in transcriptional regulation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, May 2010]