WNT8B (NM_003393) Human Tagged ORF Clone
CAT#: RG222670
- TrueORF®
WNT8B (tGFP-tagged) - Human wingless-type MMTV integration site family, member 8B (WNT8B)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_003393" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | WNT8B |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222670 representing NM_003393
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTTTCAAAGCCTTCTGTGTACATCTGTCTTTTCACCTGTGTCCTCCAACTCAGCCACAGCTGGT CGGTGAACAATTTCCTGATGACTGGTCCAAAGGCTTACCTGATTTACTCCAGCAGTGTGGCAGCTGGTGC CCAGAGTGGTATTGAAGAATGCAAGTATCAGTTTGCCTGGGACCGCTGGAACTGCCCTGAGAGAGCCCTG CAGCTGTCCAGCCATGGTGGGCTTCGCAGTGCCAATCGGGAGACAGCATTTGTGCATGCCATCAGTTCTG CTGGAGTCATGTACACCCTGACTAGAAACTGCAGCCTTGGAGATTTTGATAACTGTGGCTGTGATGACTC CCGCAACGGGCAACTGGGGGGACAAGGCTGGCTGTGGGGAGGCTGCAGTGACAATGTGGGCTTCGGAGAG GCGATTTCCAAGCAGTTTGTCGATGCCCTGGAAACAGGACAGGATGCACGGGCAGCCATGAACCTGCACA ACAACGAGGCTGGCCGCAAGGCGGTGAAGGGCACCATGAAACGCACGTGCAAGTGCCACGGCGTGTCTGG CAGCTGCACCACGCAGACCTGTTGGCTGCAGCTGCCCGAGTTCCGCGAGGTGGGCGCGCACCTGAAGGAG AAGTACCACGCAGCACTCAAGGTGGACCTGCTGCAGGGTGCTGGCAACAGCGCGGCCGGCCGCGGCGCCA TCGCCGACACCTTTCGCTCCATCTCTACCCGGGAGCTGGTGCACCTGGAGGACTCCCCGGACTACTGCCT GGAGAACAAAACGCTAGGGCTGCTGGGCACCGAAGGCCGAGAGTGCCTAAGGCGCGGGCGGGCCCTGGGT CGCTGGGAACGCCGCAGCTGCCGCCGGCTCTGCGGGGACTGCGGGCTGGCGGTGGAGGAGCGCCGGGCCG AGACCGTGTCCAGCTGCAACTGCAAGTTCCACTGGTGCTGCGCAGTCCGCTGCGAGCAGTGCCGCCGGAG GGTCACCAAGTACTTCTGTAGCCGCGCAGAGCGGCCGCGGGGGGGCGCTGCGCACAAACCCGGGAGAAAA CCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222670 representing NM_003393
Red=Cloning site Green=Tags(s) MFLSKPSVYICLFTCVLQLSHSWSVNNFLMTGPKAYLIYSSSVAAGAQSGIEECKYQFAWDRWNCPERAL QLSSHGGLRSANRETAFVHAISSAGVMYTLTRNCSLGDFDNCGCDDSRNGQLGGQGWLWGGCSDNVGFGE AISKQFVDALETGQDARAAMNLHNNEAGRKAVKGTMKRTCKCHGVSGSCTTQTCWLQLPEFREVGAHLKE KYHAALKVDLLQGAGNSAAGRGAIADTFRSISTRELVHLEDSPDYCLENKTLGLLGTEGRECLRRGRALG RWERRSCRRLCGDCGLAVEERRAETVSSCNCKFHWCCAVRCEQCRRRVTKYFCSRAERPRGGAAHKPGRK P TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003393 |
ORF Size | 1053 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003393.4 |
RefSeq Size | 2117 bp |
RefSeq ORF | 1056 bp |
Locus ID | 7479 |
UniProt ID | Q93098 |
Cytogenetics | 10q24.31 |
Domains | wnt |
Protein Families | Cancer stem cells, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway |
Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
Gene Summary | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222670 | WNT8B (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 8B (WNT8B) |
USD 457.00 |
|
RC222670L1 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 8B (WNT8B), Myc-DDK-tagged |
USD 757.00 |
|
RC222670L2 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 8B (WNT8B), mGFP tagged |
USD 757.00 |
|
RC222670L3 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 8B (WNT8B), Myc-DDK-tagged |
USD 757.00 |
|
RC222670L4 | Lenti ORF clone of Human wingless-type MMTV integration site family, member 8B (WNT8B), mGFP tagged |
USD 757.00 |
|
SC118001 | WNT8B (untagged)-Human wingless-type MMTV integration site family, member 8B (WNT8B) |
USD 457.00 |
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