TOR1AIP1 (NM_015602) Human Tagged ORF Clone

CAT#: RG221686

TOR1AIP1 (tGFP-tagged) - Human torsin A interacting protein 1 (TOR1AIP1)

Product Images

Circular map for RG221686

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TOR1AIP1
• Synonyms: LAP1; LAP1B; LAP1C; LGMD2Y
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221686 representing NM_015602
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGACGGGCGGCGGGCAGAGGCGGTGCGGGAAGGATGGGGTGTGTACGTCACCCCCAGGGCCC
  CCATCCGAGAGGGAAGGGGCCGGCTCGCCCCTCAAAATGGCGGCAGCAGCGATGCGCCTGCGTACAGAAC
  TCCTCCGTCGCGCCAGGGCCGGCGGGAAGTGAGGTTCTCGGACGAGCCGCCAGAAGTGTACGGCGACTTC
  GAGCCCCTGGTGGCCAAAGAAAGGTCCCCGGTGGGAAAACGAACCCGGCTAGAAGAGTTCCGGTCCGATT
  CTGCGAAAGAGGAAGTGAGAGAAAGCGCGTACTACCTTCGGTCTAGGCAGCGGAGGCAGCCGCGACCCCA
  GGAAACCGAGGAAATGAAGACGCGAAGGACTACCCGCCTTCAGCAGCAGCACTCAGAGCAGCCTCCGCTA
  CAGCCGTCTCCTGTTATGACCAGGAGAGGGCTGCGGGACTCTCATTCCTCTGAAGAGGATGAAGCATCTT
  CCCAAACTGATTTAAGCCAAACGATCTCAAAGAAAACTGTCAGGAGCATACAAGAGGCTCCAGTGAGTGA
  AGATCTTGTAATCAGGTTACGTCGACCCCCTCTAAGATACCCAAGATATGAAGCCACCAGTGTCCAACAG
  AAGGTCAATTTCTCTGAAGAAGGAGAAACTGAAGAAGATGATCAAGACAGCTCTCACAGCAGTGTCACTA
  CTGTTAAGGCCAGATCCAGGGATTCTGATGAATCTGGAGATAAAACCACCAGATCATCTAGTCAATATAT
  AGAATCATTTTGGCAGTCATCACAAAGTCAAAACTTCACAGCTCATGATAAGCAACCTTCAGTGCTAAGC
  TCAGGATATCAAAAAACTCCCCAGGAATGGGCCCCACAAACTGCAAGAATAAGGACCAGGATGCAAAATG
  ACAGCATTCTGAAATCAGAGCTTGGAAACCAGTCACCATCAACCTCCAGCCGACAAGTGACTGGACAACC
  CCAAAATGCATCTTTTGTCAAGAGGAACCGGTGGTGGCTACTTCCTCTGATAGCTGCTCTTGCCTCTGGG
  AGTTTTTGGTTCTTTAGTACTCCTGAGGTAGAAACCACTGCTGTTCAAGAGTTCCAGAACCAGATGAATC
  AACTTAAGAATAAGTACCAAGGTCAAGATGAGAAGCTGTGGAAAAGGAGCCAAACATTCCTGGAAAAACA
  TCTTAATAGCTCCCATCCTCGGTCTCAGCCTGCTATCTTACTGCTCACTGCTGCCCGAGATGCTGAAGAA
  GCACTTAGGTGTCTGAGTGAACAAATTGCTGATGCCTATTCTTCTTTTCGTAGTGTCCGTGCCATCCGGA
  TTGATGGGACAGATAAAGCTACTCAAGACAGTGATACTGTCAAACTAGAGGTAGACCAAGAACTGAGCAA
  TGGATTTAAGAATGGCCAGAATGCAGCTGTGGTACACCGCTTTGAGTCATTTCCCGCAGGCTCTACTTTG
  ATCTTCTACAAATATTGTGACCATGAAAACGCGGCCTTCAAAGATGTAGCCTTAGTCCTGACTGTCTTAT
  TGGAGGAAGAGACACTTGGAACAAGTCTAGGCCTAAAGGAAGTTGAAGAAAAAGTAAGAGATTTTCTTAA
  AGTCAAGTTCACCAATTCTAACACACCCAACTCCTACAATCATATGGACCCAGACAAACTGAATGGCCTC
  TGGAGCCGTATTTCTCACTTAGTTCTGCCTGTGCAACCTGAAAATGCCCTGAAAAGGGGCATCTGCTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221686 representing NM_015602
  Red=Cloning site Green=Tags(s)
  
  MAGDGRRAEAVREGWGVYVTPRAPIREGRGRLAPQNGGSSDAPAYRTPPSRQGRREVRFSDEPPEVYGDF
  EPLVAKERSPVGKRTRLEEFRSDSAKEEVRESAYYLRSRQRRQPRPQETEEMKTRRTTRLQQQHSEQPPL
  QPSPVMTRRGLRDSHSSEEDEASSQTDLSQTISKKTVRSIQEAPVSEDLVIRLRRPPLRYPRYEATSVQQ
  KVNFSEEGETEEDDQDSSHSSVTTVKARSRDSDESGDKTTRSSSQYIESFWQSSQSQNFTAHDKQPSVLS
  SGYQKTPQEWAPQTARIRTRMQNDSILKSELGNQSPSTSSRQVTGQPQNASFVKRNRWWLLPLIAALASG
  SFWFFSTPEVETTAVQEFQNQMNQLKNKYQGQDEKLWKRSQTFLEKHLNSSHPRSQPAILLLTAARDAEE
  ALRCLSEQIADAYSSFRSVRAIRIDGTDKATQDSDTVKLEVDQELSNGFKNGQNAAVVHRFESFPAGSTL
  IFYKYCDHENAAFKDVALVLTVLLEEETLGTSLGLKEVEEKVRDFLKVKFTNSNTPNSYNHMDPDKLNGL
  WSRISHLVLPVQPENALKRGICL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015602
• ORF Size: 1749 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015602.4
• RefSeq Size: 3824 bp
• RefSeq ORF: 1752 bp
• Locus ID: 26092
• UniProt ID: Q5JTV8
• Cytogenetics: 1q25.2
• Gene Summary: This gene encodes a type 2 integral membrane protein that binds A- and B-type lamins. The encoded protein localizes to the inner nuclear membrane and may be involved in maintaining the attachment of the nuclear membrane to the nuclear lamina during cell division. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2016]