CCDC142 (NM_032779) Human Tagged ORF Clone

CAT#: RG219507

CCDC142 (tGFP-tagged) - Human coiled-coil domain containing 142 (CCDC142)

Product Images

Circular map for RG219507

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CCDC142
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219507 representing NM_032779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGGCGTCTCGCTCAGGTAGCCTGCCTCCACTCGTTATCGTGCCCCCGCTGAGGGCGCAACCCG
  GGGGCACTGGGGAGGAGCAGTGGGAGAGAAGTCGAACGGGCGGTCTTCGCTGGGAGGTTCACTGCTGGCC
  GAGCGGAACTTCTGGAGGGACGCCGTGGTGGCCGACGCCGGCGGATGTGAGCGAGGACTACGAGGCTGAT
  GCTGCGGCCTGGAGGCGGGGGCCCGCAGGTGGCGGCCCGATCCCTCCCGCGCTGCAGCGTCTCCGGGCGG
  TGTTGCTGCGGCTGCATCGCGAGCGGGAGCAGCTCCTCCAGGCCCGAGACTGCGCCTACCACCTACAGTC
  GGCTGTGCGACTCATGAAGACCCTGAGTCCTGGCTCGCCATCCGGCGGCCCTAGCCCCTTGCCCCAGTGG
  TACCGCGACCTGCAGCTGCACCCTTCCCAAGGGGCGGTTCTGCGAATCGGCCCTGGGGAGACTCTCGAGC
  CGCTGCTGCTAGCGCGCCCCATCGGACTAGCCGCCCAGTGCCTGGAGGCTGTCATCGAGATGCAGCTTCG
  CGCTCTCGGCCGGGAGCCCGCCAGCCCGGGCCTGTCGTCCCAACTCGCCGAGCTGCTCTTTGCACTTCCC
  GCCTACCACACACTACAGAGAAAAGCCTTGAGCCACGTCCCAGGGGCCGCACGTCCTTTCCCCACGTCCC
  GTGTGCTCCGCCTCTTGACGGGGGAGCGGGGTTGCCAGGTGGCAAGTCGGCTGGACGAGGCGCTCCAAGG
  ATCGGCGTTGAGGGACCAGCTCCGCAGGCGGTGCCAAGAGGAGGGGGATCTGCTACCAGGGCTGCTGGGC
  CTGGTCGGGGGCGTGGCGGGTTCAGCCAGCTGTGGACTAGGGCTCGGAGGGGCTGGGGCCTTGTGGTGCC
  AATACTGGACCCTGCTGTGGGCAGCCTGTGCTCAGAGTCTGGACCTAAATCTGGGACCCTGGAGGGACCC
  CAGGGCAACAGCGCAACAGCTGAGTCAGGCACTGGGTCAGGCATCCCTGCCTCAGGAGTGTGAGAAGGAG
  CTGGCATCTTTGTGTCACAGACTACTTCATCAGTCGCTTATCTGGAGCTGGGACCAAGGTTTCTGCCAGG
  CCTTGGGATCAGCTCTTGGGGGTCAGAGCAGCCTTCCCACATCCTCTGGCACTGCTGAACTTTTGCAGCA
  GCTCTTTCCTCCTCTCTTGGATGCCCTTCGAGAGCCCAGGTTACGACGGATTTTCTGCCAGCCTGCAGGT
  CTCTGTACCCTTCAGACCACCTTGCTCTGGTTCCTGGGCAGAGCTCAGCAGTACTTGGCAGCATGGGACC
  CAGCTTCCTTCCTGCTCCTGATCCAAAAGGACTTACCTCCTCTGTTGCATGAGGCAGAAGCTTTGTATAG
  CCTGGCCTCAGAGGAAAGCTTAGCTCTGGAAGTGGAGCAGCAGCTGGGCCTGGAGATCCAGAAGCTGACT
  GCACAGATCCAGCTCCTGCCTGAAGAGTCACTAAGTGTCTTTTCTCAAGAATGTCATAAACAAGCCATGC
  AAGGTTTCAAGCTCTACATGCCACGGGGTCGGTACTGGCGGCTTCGTCTCTGTCCTGAACCTCCCAGTGC
  TCCTAGTGAGTATGCTGGTTTAGTGGTCCGCACCGTACTGGAGCCTGTGTTGCAAGGATTGCAAGGGTTG
  CCACCTCAAGCCCAGGCCCCTGCCCTTGGTCAGGCTCTGACGGCCATCGTGGGTGCCTGGCTTGACCACA
  TTCTTACCCATGGGATTCGGTTCAGCCTGCAGGGAGCGCTGCAGCTCAAACAAGACTTTGGAGTGGTCAG
  GGAGTTGCTGGAAGAGGAGCAGTGGAGCCTGTCCCCTGATCTCCGCCAGACCCTGCTCATGCTCAGCATC
  TTCCAGCAGCTGGATGGGGCCCTACTGTGTCTGTTGCAGCAGCCCCTGCCCAAGTCTCAAGTCCACAGGA
  GGCCCCCCTGTTGCTGTGCTTGTCAGGAGGTCCAGACCACGAAATTGCCCAGCAGCTGCCTCAATAGCCT
  GGAGAGCTTGGAGCCCCCGCTCCAGCCTGGAACATCTCCAGCCCAGACAGGTCAGCTGCAAAGCACACTA
  GGAGGAAGGGGACCTAGCCCGGAGGGCTACCTGGTGGGAAATCAGCAGGCCTGGCTTGCCCTCAGGCAAC
  ACCAGCGACCCCGTTGGCACCTGCCGTTTTTTTCCTGCCTGGGAACCAGTCCTGAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219507 representing NM_032779
  Red=Cloning site Green=Tags(s)
  
  MAQASRSGSLPPLVIVPPLRAQPGGTGEEQWERSRTGGLRWEVHCWPSGTSGGTPWWPTPADVSEDYEAD
  AAAWRRGPAGGGPIPPALQRLRAVLLRLHREREQLLQARDCAYHLQSAVRLMKTLSPGSPSGGPSPLPQW
  YRDLQLHPSQGAVLRIGPGETLEPLLLARPIGLAAQCLEAVIEMQLRALGREPASPGLSSQLAELLFALP
  AYHTLQRKALSHVPGAARPFPTSRVLRLLTGERGCQVASRLDEALQGSALRDQLRRRCQEEGDLLPGLLG
  LVGGVAGSASCGLGLGGAGALWCQYWTLLWAACAQSLDLNLGPWRDPRATAQQLSQALGQASLPQECEKE
  LASLCHRLLHQSLIWSWDQGFCQALGSALGGQSSLPTSSGTAELLQQLFPPLLDALREPRLRRIFCQPAG
  LCTLQTTLLWFLGRAQQYLAAWDPASFLLLIQKDLPPLLHEAEALYSLASEESLALEVEQQLGLEIQKLT
  AQIQLLPEESLSVFSQECHKQAMQGFKLYMPRGRYWRLRLCPEPPSAPSEYAGLVVRTVLEPVLQGLQGL
  PPQAQAPALGQALTAIVGAWLDHILTHGIRFSLQGALQLKQDFGVVRELLEEEQWSLSPDLRQTLLMLSI
  FQQLDGALLCLLQQPLPKSQVHRRPPCCCACQEVQTTKLPSSCLNSLESLEPPLQPGTSPAQTGQLQSTL
  GGRGPSPEGYLVGNQQAWLALRQHQRPRWHLPFFSCLGTSPES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032779
• ORF Size: 2229 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032779.2, NP_116168.2
• RefSeq Size: 2835 bp
• RefSeq ORF: 2232 bp
• Locus ID: 84865
• UniProt ID: Q17RM4
• Cytogenetics: 2p13.1