Integrin alpha E (ITGAE) (NM_002208) Human Tagged ORF Clone

CAT#: RG217265

ITGAE (tGFP-tagged) - Human integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) (ITGAE)

Product Images

Circular map for RG217265

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Integrin alpha E
• Synonyms: CD103; HUMINAE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG217265 representing NM_002208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCTTCCACACTCTGCTCTGCATAGCCAGCCTGGCCCTGCTGGCCGCTTTCAATGTGGATGTGG
  CCCGGCCCTGGCTCACGCCCAAGGGAGGTGCCCCTTTCGTGCTCAGCTCCCTTCTGCACCAAGACCCCAG
  CACCAACCAGACCTGGCTCCTGGTCACCAGCCCCAGAACCAAGAGGACACCAGGGCCCCTCCATCGATGT
  TCCCTTGTCCAGGATGAAATCCTTTGCCATCCTGTAGAGCATGTCCCCATCCCCAAGGGGAGGCACCGGG
  GAGTGACCGTTGTCCGGAGCCACCACGGTGTTTTGATATGCATTCAAGTGCTGGTCCGGCGGCCTCACAG
  CCTCAGCTCAGAACTCACAGGCACCTGTAGCCTCCTGGGCCCTGACCTCCGTCCCCAGGCTCAGGCCAAC
  TTCTTCGACCTTGAAAATCTCCTGGATCCAGATGCACGTGTGGACACTGGAGACTGCTACAGCAACAAAG
  AAGGCGGTGGAGAAGACGATGTGAACACAGCCAGGCAGCGCCGGGCTCTGGAGAAGGAGGAGGAGGAAGA
  CAAGGAGGAGGAGGAAGACGAGGAGGAGGAGGAAGCTGGCACCGAGATTGCCATCATCCTGGATGGCTCA
  GGAAGCATTGATCCCCCAGACTTTCAGAGAGCCAAAGACTTCATCTCCAACATGATGAGGAACTTCTATG
  AAAAGTGTTTTGAGTGCAACTTTGCCTTGGTGCAGTATGGAGGAGTGATCCAGACTGAGTTTGACCTTCG
  GGACAGCCAGGATGTGATGGCCTCCCTCGCCAGAGTCCAGAACATCACTCAAGTGGGGAGTGTCACCAAG
  ACTGCCTCAGCCATGCAACACGTCTTAGACAGCATCTTCACCTCAAGCCACGGCTCCAGGAGAAAGGCAT
  CCAAGGTCATGGTGGTGCTCACCGATGGTGGCATATTCGAGGACCCCCTCAACCTTACGACAGTCATCAA
  CTCCCCCAAAATGCAGGGTGTTGAGCGCTTTGCCATTGGGGTGGGAGAAGAATTTAAGAGTGCTAGGACT
  GCGAGGGAACTGAACCTGATCGCCTCAGACCCGGATGAGACCCATGCTTTCAAGGTGACCAACTACATGG
  CGCTGGATGGGCTGCTGAGCAAACTGCGGTACAACATCATCAGCATGGAAGGCACGGTTGGAGACGCCCT
  TCACTACCAGCTGGCACAGATTGGCTTCAGTGCTCAGATCCTGGATGAGCGGCAGGTGCTGCTCGGCGCC
  GTCGGGGCCTTTGACTGGTCCGGAGGGGCGTTGCTCTACGACACACGCAGCCGCCGGGGCCGCTTCCTGA
  ACCAGACAGCGGCGGCGGCGGCAGACGCGGAGGCTGCGCAGTACAGCTACCTGGGTTACGCTGTGGCCGT
  GCTGCACAAGACCTGCAGCCTCTCCTACGTCGCGGGGGCTCCACAGTACAAACATCATGGGGCCGTGTTT
  GAGCTCCAGAAGGAGGGCAGAGAGGCCAGCTTCCTGCCAGTGCTGGAGGGAGAGCAGATGGGGTCCTATT
  TTGGCTCTGAGCTGTGCCCTGTGGACATTGACATGGATGGAAGCACGGACTTCTTGCTGGTGGCTGCTCC
  ATTTTACCACGTTCATGGAGAAGAAGGCAGAGTCTACGTGTACCGTCTCAGCGAGCAGGATGGTTCTTTC
  TCCTTGGCACGCATACTGAGTGGGCACCCCGGGTTCACCAATGCCCGCTTTGGCTTTGCCATGGCGGCTA
  TGGGGGATCTCAGTCAGGATAAGCTCACAGATGTGGCCATCGGGGCCCCCCTGGAAGGTTTTGGGGCAGA
  TGATGGTGCCAGCTTCGGCAGTGTGTATATCTACAATGGACACTGGGACGGCCTCTCCGCCAGCCCCTCG
  CAGCGGATCAGAGCCTCCACGGTGGCCCCAGGACTCCAGTACTTCGGCATGTCCATGGCTGGTGGCTTTG
  ATATTAGTGGCGACGGCCTTGCCGACATCACCGTGGGCACTCTGGGCCAGGCGGTTGTGTTCCGCTCCCG
  GCCTGTGGTTCGCCTGAAGGTCTCCATGGCCTTCACCCCCAGCGCACTGCCCATCGGCTTCAACGGCGTC
  GTGAATGTCCGTTTATGTTTTGAAATCAGCTCTGTAACCACAGCCTCTGAGTCAGGCCTCCGTGAGGCAC
  TTCTCAACTTCACGCTGGATGTGGATGTGGGGAAGCAGAGGAGACGGCTGCAGTGTTCAGACGTAAGAAG
  CTGTCTGGGCTGCCTGAGGGAGTGGAGCAGCGGATCCCAGCTTTGTGAGGACCTCCTGCTCATGCCCACA
  GAGGGAGAGCTCTGTGAGGAGGACTGCTTCTCCAATGCCAGTGTCAAAGTCAGCTACCAGCTCCAGACCC
  CTGAGGGACAGACGGACCATCCCCAGCCCATCCTGGACCGCTACACTGAGCCCTTTGCCATCTTCCAGCT
  GCCCTATGAGAAGGCCTGCAAGAATAAGCTGTTTTGTGTCGCAGAATTACAGTTGGCCACCACCGTCTCT
  CAGCAGGAGTTGGTGGTGGGTCTCACAAAGGAGCTGACCCTGAACATTAACCTAACTAACTCCGGGGAAG
  ATTCCTACATGACAAGCATGGCCTTGAATTACCCCAGAAACCTGCAGTTGAAGAGGATGCAAAAGCCTCC
  CTCTCCAAACATTCAGTGTGATGACCCTCAGCCGGTTGCTTCTGTCCTGATCATGAACTGCAGGATTGGT
  CACCCCGTCCTCAAGAGGTCATCTGCTCATGTTTCAGTCGTTTGGCAGCTAGAGGAGAATGCCTTTCCAA
  ACAGGACAGCAGACATCACTGTGACTGTCACCAATTCCAATGAAAGACGGTCTTTGGCCAACGAGACCCA
  CACCCTTCAATTCAGGCATGGCTTCGTTGCAGTTCTGTCCAAACCATCCATAATGTACGTGAACACAGGC
  CAGGGGCTTTCTCACCACAAAGAATTCCTCTTCCATGTACATGGGGAGAACCTCTTTGGAGCAGAATACC
  AGTTGCAAATTTGCGTCCCAACCAAATTACGAGGTCTCCAGGTTGTAGCAGTGAAGAAGCTGACGAGGAC
  TCAGGCCTCCACGGTGTGCACCTGGAGTCAGGAGCGCGCTTGTGCGTACAGTTCGGTTCAGCATGTGGAA
  GAATGGCATTCAGTGAGCTGTGTCATCGCTTCAGATAAAGAAAATGTCACCGTGGCTGCAGAGATCTCCT
  GGGATCACTCTGAGGAGTTACTAAAAGATGTAACTGAACTGCAGATCCTTGGTGAAATATCTTTCAACAA
  ATCTCTATATGAGGGACTGAATGCAGAGAACCACAGAACTAAGATCACTGTCGTCTTCCTGAAAGATGAG
  AAGTACCATTCTTTGCCTATCATCATTAAAGGCAGCGTTGGTGGACTTCTGGTGTTGATCGTGATTCTGG
  TCATCCTGTTCAAGTGTGGCTTTTTTAAAAGAAAATATCAACAACTGAACTTGGAGAGCATCAGGAAGGC
  CCAGCTGAAATCAGAGAATCTGCTCGAAGAAGAGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG217265 representing NM_002208
  Red=Cloning site Green=Tags(s)
  
  MWLFHTLLCIASLALLAAFNVDVARPWLTPKGGAPFVLSSLLHQDPSTNQTWLLVTSPRTKRTPGPLHRC
  SLVQDEILCHPVEHVPIPKGRHRGVTVVRSHHGVLICIQVLVRRPHSLSSELTGTCSLLGPDLRPQAQAN
  FFDLENLLDPDARVDTGDCYSNKEGGGEDDVNTARQRRALEKEEEEDKEEEEDEEEEEAGTEIAIILDGS
  GSIDPPDFQRAKDFISNMMRNFYEKCFECNFALVQYGGVIQTEFDLRDSQDVMASLARVQNITQVGSVTK
  TASAMQHVLDSIFTSSHGSRRKASKVMVVLTDGGIFEDPLNLTTVINSPKMQGVERFAIGVGEEFKSART
  ARELNLIASDPDETHAFKVTNYMALDGLLSKLRYNIISMEGTVGDALHYQLAQIGFSAQILDERQVLLGA
  VGAFDWSGGALLYDTRSRRGRFLNQTAAAAADAEAAQYSYLGYAVAVLHKTCSLSYVAGAPQYKHHGAVF
  ELQKEGREASFLPVLEGEQMGSYFGSELCPVDIDMDGSTDFLLVAAPFYHVHGEEGRVYVYRLSEQDGSF
  SLARILSGHPGFTNARFGFAMAAMGDLSQDKLTDVAIGAPLEGFGADDGASFGSVYIYNGHWDGLSASPS
  QRIRASTVAPGLQYFGMSMAGGFDISGDGLADITVGTLGQAVVFRSRPVVRLKVSMAFTPSALPIGFNGV
  VNVRLCFEISSVTTASESGLREALLNFTLDVDVGKQRRRLQCSDVRSCLGCLREWSSGSQLCEDLLLMPT
  EGELCEEDCFSNASVKVSYQLQTPEGQTDHPQPILDRYTEPFAIFQLPYEKACKNKLFCVAELQLATTVS
  QQELVVGLTKELTLNINLTNSGEDSYMTSMALNYPRNLQLKRMQKPPSPNIQCDDPQPVASVLIMNCRIG
  HPVLKRSSAHVSVVWQLEENAFPNRTADITVTVTNSNERRSLANETHTLQFRHGFVAVLSKPSIMYVNTG
  QGLSHHKEFLFHVHGENLFGAEYQLQICVPTKLRGLQVVAVKKLTRTQASTVCTWSQERACAYSSVQHVE
  EWHSVSCVIASDKENVTVAAEISWDHSEELLKDVTELQILGEISFNKSLYEGLNAENHRTKITVVFLKDE
  KYHSLPIIIKGSVGGLLVLIVILVILFKCGFFKRKYQQLNLESIRKAQLKSENLLEEEN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002208
• ORF Size: 3537 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002208.4, NP_002199.3
• RefSeq Size: 3878 bp
• RefSeq ORF: 3540 bp
• Locus ID: 3682
• UniProt ID: P38570
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]