SNRPF (NM_003095) Human Tagged ORF Clone
CAT#: RG200416
- TrueORF®
SNRPF (tGFP-tagged) - Human small nuclear ribonucleoprotein polypeptide F (SNRPF)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_003095" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | SNRPF |
Synonyms | Sm-F; SMF; snRNP-F |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200416 representing NM_003095
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTTACCCCTCAATCCCAAACCTTTCCTCAATGGACTAACAGGAAAGCCAGTGATGGTGAAACTTA AGTGGGGAATGGAGTACAAGGGCTATCTGGTATCTGTAGATGGCTACATGAACATGCAGCTTGCAAATAC AGAAGAATACATAGATGGAGCTTTGTCTGGACATCTGGGTGAAGTTTTAATAAGGTGTAATAATGTCCTT TATATCAGAGGTGTGGAAGAAGAGGAAGAAGATGGGGAAATGAGAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200416 representing NM_003095
Red=Cloning site Green=Tags(s) MSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVL YIRGVEEEEEDGEMRE TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003095 |
ORF Size | 258 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003095.3 |
RefSeq Size | 806 bp |
RefSeq ORF | 261 bp |
Locus ID | 6636 |
UniProt ID | P62306 |
Cytogenetics | 12q23.1 |
Domains | Sm |
Protein Families | Stem cell - Pluripotency |
Protein Pathways | Spliceosome |
Gene Summary | Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:23333303, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346). Is also a component of the minor U12 spliceosome (PubMed:15146077). As part of the U7 snRNP it is involved in histone 3'-end processing (PubMed:12975319).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200416 | SNRPF (Myc-DDK-tagged)-Human small nuclear ribonucleoprotein polypeptide F (SNRPF) |
USD 150.00 |
|
RC200416L3 | Lenti ORF clone of Human small nuclear ribonucleoprotein polypeptide F (SNRPF), Myc-DDK-tagged |
USD 450.00 |
|
RC200416L4 | Lenti ORF clone of Human small nuclear ribonucleoprotein polypeptide F (SNRPF), mGFP tagged |
USD 450.00 |
|
SC108475 | SNRPF (untagged)-Human small nuclear ribonucleoprotein polypeptide F (SNRPF) |
USD 150.00 |
|
SC320607 | SNRPF (untagged)-Human small nuclear ribonucleoprotein polypeptide F (SNRPF) |
USD 150.00 |
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